Li Luya, Feng Xue, Chen Yuting, Li Shenghao, Sun Yupeng, Zhang Lantong
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University Shijiazhuang 050017 P. R. China
Department of Pathobiology and Immunology, Hebei University of Chinese Medicine Shijiazhuang 050000 P. R. China.
RSC Adv. 2019 Aug 12;9(43):24963-24980. doi: 10.1039/c9ra03037a. eCollection 2019 Aug 8.
Eriocitrin, a main flavonoid in lemons, possesses strong antioxidant, lipid-lowering and anticancer activities and has long been used in food, beverages and wine. However, its metabolism and is still unclear. In this study, an efficient strategy was developed to detect and identify metabolites of eriocitrin by using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) based on online data acquisition and multiple data processing techniques. A total of 32 metabolites and 27 metabolites were obtained based on the above method. Furthermore, the main metabolic pathways of eriocitrin included reduction, hydrogenation, -acetylation, ketone formation, oxidation, methylation, sulfate conjugation, glutamine conjugation, glycine conjugation, desaturation and demethylation to carboxylic acid. This study will lay a foundation for further studies on the metabolic mechanisms of eriocitrin.
橙皮苷是柠檬中的主要黄酮类化合物，具有强大的抗氧化、降血脂和抗癌活性，长期以来一直用于食品、饮料和葡萄酒中。然而，其代谢情况仍不清楚。在本研究中，基于在线数据采集和多种数据处理技术，开发了一种利用超高效液相色谱-混合三重四极杆飞行时间质谱联用（UHPLC-Q-TOF-MS）检测和鉴定橙皮苷代谢物的有效策略。基于上述方法共获得了32种代谢物和27种代谢物。此外，橙皮苷的主要代谢途径包括还原、氢化、乙酰化、酮形成、氧化、甲基化、硫酸结合、谷氨酰胺结合、甘氨酸结合、去饱和以及脱甲基生成羧酸。本研究将为进一步研究橙皮苷的代谢机制奠定基础。
