Xavier Aline, Zacconi Flavia, Gainza Constanza, Cabrera Daniel, Arrese Marco, Uribe Sergio, Sing-Long Carlos, Andia Marcelo E
Biomedical Imaging Center, Pontificia Universidad Católica de Chile Chile
Millennium Nucleus for Cardiovascular Magnetic Resonance Chile.
RSC Adv. 2019 Dec 19;9(72):42132-42139. doi: 10.1039/c9ra08914d. eCollection 2019 Dec 18.
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease in the world and it is becoming one of the most frequent cause of liver transplantation. Unfortunately, the only available method that can reliably determine the stage of this disease is liver biopsy, however, it is invasive and risky for patients. The purpose of this study is to investigate changes in the intracellular composition of the liver fatty acids during the progression of the NAFLD in a mouse model fed with Western diet, with the aim of identify non-invasive biomarkers of NAFLD progression based in H-MRS. Our results showed that the intracellular liver fatty acid composition changes as NAFLD progresses from simple steatosis to steatohepatitis (NASH). Using principal component analysis with a clustering method, it was possible to identify the three most relevant clinical groups: normal, steatosis and NASH by using H-MRS. These results showed a good agreement with the results obtained by GC-MS and histology. Our results suggest that it would be possible to detect the progression of simple steatosis to NASH using H-MRS, that has the potential to be used routinely in clinical application for screening high-risk patients.
非酒精性脂肪性肝病（NAFLD）是全球最常见的肝脏疾病，并且正成为肝移植最常见的病因之一。不幸的是，唯一能够可靠确定该疾病阶段的方法是肝活检，然而，这对患者具有侵入性且有风险。本研究的目的是在喂食西方饮食的小鼠模型中，研究NAFLD进展过程中肝脏脂肪酸细胞内组成的变化，旨在基于氢磁共振波谱（H-MRS）识别NAFLD进展的非侵入性生物标志物。我们的结果表明，随着NAFLD从单纯性脂肪变性进展为脂肪性肝炎（NASH），肝脏脂肪酸的细胞内组成会发生变化。使用主成分分析和聚类方法，通过H-MRS能够识别出三个最相关的临床组：正常、脂肪变性和NASH。这些结果与通过气相色谱-质谱联用仪（GC-MS）和组织学获得的结果高度一致。我们的结果表明，使用H-MRS有可能检测到单纯性脂肪变性向NASH的进展，其有潜力在临床应用中常规用于筛查高危患者。
