USDA-ARS, Cereal Disease Laboratory, 1551 Lindig St., St. Paul, MN, 55108, USA.
Plant Genome. 2022 Sep;15(3):e20215. doi: 10.1002/tpg2.20215. Epub 2022 May 11.
The winter wheat (Triticum aestivum L.) line CI13227 has been characterized as having adult plant resistance to leaf rust caused by Puccinia triticina Eriks (Pt). Line CI13227 was crossed with the susceptible spring wheat 'Thatcher' (Tc) and a Tc*2/CI13227 F line with adult plant leaf rust resistance designated as 411A was derived. Line 411A was crossed with Tc to develop an F recombinant inbred line (RIL) population. The parents and 120 F lines were assessed for leaf rust severity at the flag leaf stage in five field plot tests from 2011 through 2015 and were genotyped for single-nucleotide polymorphism (SNP) markers with the Illumina iSelect 90K wheat bead array. A total of 2,384 SNP markers segregated among the RILs. Completely linked SNPs were removed, and 474 markers that covered 2,605 centimorgans (cM) were used for linkage map construction. Quantitative trait loci (QTL) on chromosome 2AL with logarithm of odds (LOD) values 2.34-7.88, on chromosome 4BS with LOD values 1.35- 4.66, and on chromosome 7AL with LOD values 2.92-7.81 were associated with significant reduction in leaf rust severity in the field plot tests. Recombinant inbred lines that had combinations of two or three of the QTL had significantly lower leaf rust severity than RILs that lacked any resistance QTL. Kompetitive allele specific polymerase chain reaction (KASP) markers were developed for the SNPs that were closely linked with the three QTL to facilitate marker-based selection of the leaf rust resistance in breeding programs.
冬小麦（Triticum aestivum L.）品系 CI13227 被鉴定为具有成株期抗叶锈病的特性，由 Puccinia triticina Eriks（Pt）引起。该品系与感病的春小麦“撒切尔”（Tc）杂交，并衍生出一个具有成株期叶锈病抗性的 Tc*2/CI13227 F 系，命名为 411A。411A 与 Tc 杂交，开发了一个 F 重组自交系（RIL）群体。亲本和 120 个 F 系在 2011 年至 2015 年的五个田间试验中，在旗叶期对叶锈病严重程度进行了评估，并利用 Illumina iSelect 90K 小麦珠阵列对单核苷酸多态性（SNP）标记进行了基因型分析。共有 2384 个 SNP 标记在 RIL 中分离。去除完全连锁的 SNP，选择覆盖 2605 厘摩尔（cM）的 474 个标记用于连锁图谱构建。在染色体 2AL 上，对数优势（LOD）值为 2.34-7.88 的 QTL，在染色体 4BS 上，LOD 值为 1.35-4.66 的 QTL，在染色体 7AL 上，LOD 值为 2.92-7.81 的 QTL 与田间试验中叶锈病严重程度的显著降低有关。与缺乏任何抗性 QTL 的 RIL 相比，具有两个或三个 QTL 组合的重组自交系的叶锈病严重程度显著降低。为与三个 QTL 紧密连锁的 SNP 开发了竞争性等位基因特异聚合酶链反应（KASP）标记，以促进在育种计划中对叶锈病抗性的标记辅助选择。
