Cereal Disease Laboratory, U.S. Department of Agriculture Agricultural Research Service, St. Paul, MN 55108.
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108.
Phytopathology. 2021 Feb;111(2):380-385. doi: 10.1094/PHYTO-03-20-0074-R. Epub 2020 Dec 30.
AC Taber is a hard red spring wheat cultivar that has had long-lasting resistance to the leaf rust fungus . The objective of this study was to determine the chromosome location of the leaf rust resistance genes in AC Taber. The leaf rust-susceptible cultivar Thatcher was crossed with AC Taber to develop an F recombinant inbred line (RIL) population. The RILs and parents were evaluated for segregation of leaf rust resistance in five field plot tests and in two seedling tests to race BBBDB of . A genetic map of the RIL population was developed using 90,000 single nucleotide polymorphism markers with the Illumina Infinium iSelect 90K wheat bead array. Quantitative trait loci (QTLs) with significant effects for lower leaf rust severity in the field plot tests were found on chromosomes 2BS and 3BS. The same QTLs also had significant effects for lower infection type in seedlings to leaf rust race BBBDB. The gene on 2BS was the adult plant resistance gene , and the gene on 3BS mapped to the same region as the adult plant resistance gene and other QTLs for leaf rust resistance. Kompetitive allele-specific PCR assay markers linked to the 2BS and 3BS regions were developed and should be useful for marker-based selection of these genes.
AC 塔博尔是一个硬红春小麦品种，对叶锈病真菌具有持久的抗性。本研究的目的是确定 AC 塔博尔中叶锈病抗性基因的染色体位置。将感叶锈病的品种撒切尔与 AC 塔博尔杂交，开发了一个 F 重组自交系（RIL）群体。在五个田间小区试验和两个幼苗试验中，评估 RILs 和亲本对叶锈病的分离，以鉴定 BBBDB 小种。使用 Illumina Infinium iSelect 90K 小麦珠阵列的 90,000 个单核苷酸多态性标记开发了 RIL 群体的遗传图谱。在田间小区试验中，对叶片严重度具有显著影响的数量性状位点（QTLs）位于 2BS 和 3BS 染色体上。相同的 QTLs 对幼苗对叶锈病 BBBDB 小种的感染类型也有显著影响。2BS 上的基因是成株期抗性基因，3BS 上的基因与成株期抗性基因和其他叶锈病抗性 QTL 位于同一区域。开发了与 2BS 和 3BS 区域相关的竞争等位基因特异性 PCR 检测标记，这些标记应该对这些基因的基于标记的选择有用。
