Technical Services, Hardy Diagnostics, 1430 West McCoy Lane, Santa Maria, CA 93455, USA.
Marketing, Hardy Diagnostics, 1430 West McCoy Lane, Santa Maria, CA 93455, USA.
J AOAC Int. 2022 Oct 26;105(6):1663-1670. doi: 10.1093/jaoacint/qsac051.
CompactDry™ Yeast/Mold Rapid (YMR) is a ready-to-use dry media sheet using a chromogenic medium with selective agents for the enumeration of yeasts and molds in a variety of food products after incubation at 25 ± 1°C for 3 days. The method is certified as AOAC Performance Tested MethodSM 092002.
The CompactDry YMR method was validated for a matrix extension to cannabis flower through the AOAC Emergency Response Validation process.
The performance of the CompactDry YMR was compared to Dichloran Rose Bengal Chloramphenicol (DRBC) agar for the enumeration of yeasts and molds in cannabis flower. Matrix data were normalized by log10 transformation, and performance indicators included repeatability, difference of means (DOM), and inclusivity/exclusivity.
The results demonstrated that the CompactDry YMR method is equivalent to the DRBC agar method at 72 h of incubation. In the independent laboratory validation study, there was no significant difference in detection, enumeration, or repeatability between the CompactDry YMR method and DRBC agar at 72 h. Eighteen inclusivity and 16 exclusivity strains specific to cannabis plant materials that were not evaluated in the original CompactDry YMR method validation were tested in this study. All inclusivity organisms produced typical colonies on the CompactDry YMR. The two exclusivity bacterial strains that showed growth on CompactDry YMR at 72 h were inoculated at a high concentration.
CompactDry YMR is equivalent in performance to traditional culture media detection methods of yeasts and molds.
CompactDry YMR will streamline dried cannabis flower testing.
CompactDry™ 酵母/霉菌快速检测试剂盒（YMR）是一种即用型干燥培养基片，采用显色培养基，含有选择性添加剂，可在 25±1°C 下培养 3 天后，用于各种食品中酵母菌和霉菌的计数。该方法已通过 AOAC 性能测试方法 SM 092002 认证。
通过 AOAC 应急响应验证程序，对 CompactDry YMR 方法进行了基质扩展到大麻花的验证。
将 CompactDry YMR 的性能与孟加拉玫瑰红氯霉酚（DRBC）琼脂进行比较，用于大麻花中酵母菌和霉菌的计数。通过对数转换对基质数据进行归一化，性能指标包括重复性、均值差（DOM）和包容性/排他性。
结果表明，在 72 小时的培养时间内，CompactDry YMR 方法与 DRBC 琼脂方法等效。在独立实验室验证研究中，在 72 小时时，CompactDry YMR 方法与 DRBC 琼脂在检测、计数和重复性方面没有显著差异。本研究测试了 18 种与大麻植物材料相关但未在原始 CompactDry YMR 方法验证中评估的包容性菌株和 16 种排他性菌株。所有包容性生物体在 CompactDry YMR 上均产生典型菌落。在 72 小时时在 CompactDry YMR 上显示生长的两种排他性细菌菌株以高浓度接种。
CompactDry YMR 的性能与传统的酵母菌和霉菌培养检测方法相当。
CompactDry YMR 将简化干燥大麻花的测试。
