Le Quynh-Nhi, Montei Carolyn, Mozola Mark, Roman Brooke, Biswas Preetha, Donofrio Robert
Neogen Corp, 620 Lesher Place, Lansing, MI 48912, USA.
J AOAC Int. 2022 Apr 27;105(3):848-854. doi: 10.1093/jaoacint/qsab135.
Soleris® Direct Yeast and Mold (DYM) is a growth-based, automated method for detection of yeast and mold in select foods and other sample types including nutraceuticals and cosmetics. The Soleris method is used in a "dilute-to-specification" or threshold manner in which a result is scored as positive or negative around a predetermined cutoff (in CFU/g) established by the dilution and volume of sample homogenate tested.
The objective of this study was to validate the method for testing of dried cannabis flower. The validation was conducted under the Emergency Response Validation program of the AOAC Research Institute.
The study included inclusivity and exclusivity testing, in particular testing of yeast and mold species associated with cannabis, and a matrix study in which Soleris method presumptive results were compared with Soleris confirmed results using Dichloran Rose-Bengal Chloramphenicol (DRBC) agar for confirmation. Samples at four different levels of natural yeast and mold contamination were tested at two test thresholds.
In inclusivity testing, all 63 yeast and mold strains tested produced positive results within the specified test duration of 72 h. In exclusivity testing, 36 of 37 strains tested produced no detection within 72 h. In matrix testing, there were no significant differences between Soleris presumptive and confirmed results for any contamination level or test threshold as determined by probability of detection analysis.
Results indicate that the Soleris method is an effective procedure for detection of yeast and mold in dried cannabis flower.
With the Soleris method, results are available within 72 h compared with the 5-7 days required for microbiological culture methods.
Soleris®直接酵母和霉菌（DYM）检测系统是一种基于生长的自动化方法，用于检测特定食品及其他样品类型（包括营养保健品和化妆品）中的酵母和霉菌。Soleris检测方法采用“稀释至规格”或阈值方式，根据所测试样品匀浆的稀释度和体积确定的预定临界值（以CFU/g计），将结果判定为阳性或阴性。
本研究旨在验证该方法用于检测干燥大麻花朵的有效性。验证工作在AOAC研究所的应急响应验证计划下进行。
该研究包括适用性和专属性测试，特别是对与大麻相关的酵母和霉菌种类的测试，以及一项基质研究，即将Soleris方法的初步结果与使用孟加拉红氯霉素（DRBC）琼脂进行确证的Soleris确证结果进行比较。对四个不同天然酵母和霉菌污染水平的样品在两个测试阈值下进行检测。
在适用性测试中，所有63株测试的酵母和霉菌菌株在规定的72小时测试时间内均产生阳性结果。在专属性测试中，37株测试菌株中的36株在72小时内未检测到。在基质测试中，根据检测概率分析，对于任何污染水平或测试阈值，Soleris初步结果和确证结果之间均无显著差异。
结果表明，Soleris方法是检测干燥大麻花朵中酵母和霉菌的有效方法。
使用Soleris方法，72小时内即可获得结果，而微生物培养方法则需要5 - 7天。
