State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, PR China.
University of Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005361.
An actinobacterium, designated strain EGI L10124, was isolated from saline lake sediment collected in Xinjiang province, PR China. The taxonomic position of the isolate was determined based on polyphasic taxonomic and phylogenomic analyses. Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that strain EGI L10124 formed a distinct clade with SYSU G02662, with a shared sequence identity of 95.2 %. The novel isolate could be distinguished from species in the genus by its distinct phenotypic, physiological and genotypic characteristics. The cells of strain EGI L10124 were aerobic, Gram-stain-positive and short rod-shaped. Optimal growth conditions of strain EGI L10124 on marine agar 2216 were registered at pH 8.0 at 37 °C. In addition, -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major respiratory quinone was MK-9 (H), while the major fatty acids were iso-C, C and C. The polar lipids included diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. Based on the genome sequence of strain EGI L10124, it appears that the G+C content of the novel isolate was 71.8 mol%. According to our data, strain EGI L10124 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain of the proposed novel isolate is EGI L10124 (=CGMCC 1.19113=KCTC 49679).
一株放线菌，命名为 EGI L10124 菌株，从中国新疆盐湖沉积物中分离得到。该分离株的分类地位是基于多相分类和系统发育基因组学分析确定的。系统发育分析和 16S rRNA 基因序列相似性表明，EGI L10124 菌株与 SYSU G02662 形成一个独特的分支，序列同源性为 95.2%。新分离株可通过其独特的表型、生理和遗传特征与属内其他种区分开来。EGI L10124 菌株的细胞为需氧、革兰氏阳性、短杆状。在海洋琼脂 2216 上，EGI L10124 菌株的最佳生长条件为 pH 8.0，温度为 37°C。此外，-二氨基庚二酸是细胞壁肽聚糖中的特征性二氨基庚二酸。主要呼吸醌为 MK-9 (H)，主要脂肪酸为 iso-C15:0、C16:0 和 C17:0。极性脂包括双磷脂酰甘油、磷脂酰肌醇甘露糖苷和磷脂酰肌醇。根据 EGI L10124 菌株的基因组序列，该新分离株的 G+C 含量约为 71.8mol%。根据我们的数据，EGI L10124 菌株代表了属的一个新种，建议命名为 sp. nov.。提议的新分离株的模式菌株为 EGI L10124（=CGMCC 1.19113=KCTC 49679）。
