Gao Lei, Ma Jin-Biao, Huang Yin, Muhammad Murad, Lian Hai-Ting, Shurigin Vyacheslav, Egamberdieva Dilfuza, Li Wen-Jun, Li Li
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2024 Aug 29;15:1447755. doi: 10.3389/fmicb.2024.1447755. eCollection 2024.
This study utilized high-throughput sequencing to investigate endophytic bacteria diversity in halophytic plants (AT) and (AE) from the Aral Sea region. Following sequence processing, 356 Amplicon Sequence Variants (ASVs) were discovered. The abundance and variety of endophytic bacteria were higher in AT. , , , and constituted the dominant in AE, whereas , , , and constituted the dominant in AT. Biomarkers were identified through LEFSe analysis, showing host-specific patterns. PCoA indicated distinct bacterial community structures. Phylogenetic analysis revealed diverse endophytic bacteria, including potential novel taxa. PICRUSt2 predicted diverse functions for endophytic bacteria in halophytes, indicating recruitment of beneficial bacterial taxa to adapt to extreme hypersaline conditions, including plant growth-promoting, biocontrol, and halophilic/tolerant bacteria. Moreover, the evolutionary relationship, metabolic capabilities, and plant beneficial potentials of the strains, previously isolated from the above two halophytes, were analyzed using comparative genomic and physiological analysis. The . strains displayed versatile environmental adaptability, as shown by their ability to use a wide range of carbon sources and their salt tolerances. . possessed a wide range of enzymatic capabilities, including but not limited to proteases, cellulases, and chitinases. Comparative genomic analysis revealed that despite some variations, they shared genetic similarities and metabolic capabilities among the strains. . strains also displayed outstanding plant-growth-promoting and antagonistic potentials, offering potential solutions to the global food crisis. This study enhances our understanding of microbial diversity in halophytes on saline-alkali land in the West Aral Sea, shedding light on the halophyte microbiome and its collaboration with hosts in highly hypersaline environments. This study also provides a scientific basis for developing high-quality microbial fertilizers and implementing sustainable agricultural practices.
本研究利用高通量测序技术，对咸海地区盐生植物（AT）和（AE）中的内生细菌多样性进行了调查。经过序列处理，共发现了356个扩增子序列变体（ASV）。AT中内生细菌的丰度和种类更高。在AE中，、、、为优势菌，而在AT中，、、、为优势菌。通过LEfSe分析确定了生物标志物，显示出宿主特异性模式。主坐标分析（PCoA）表明细菌群落结构不同。系统发育分析揭示了多种内生细菌，包括潜在的新分类群。PICRUSt2预测了盐生植物中内生细菌的多种功能，表明有益细菌类群被招募以适应极端高盐环境，包括促进植物生长、生物防治以及嗜盐/耐盐细菌。此外，还利用比较基因组学和生理学分析，对先前从上述两种盐生植物中分离出的菌株的进化关系、代谢能力和植物有益潜力进行了分析。这些菌株表现出广泛的环境适应性，例如能够利用多种碳源以及具有耐盐性。拥有广泛的酶促能力，包括但不限于蛋白酶、纤维素酶和几丁质酶。比较基因组分析表明，尽管存在一些差异，但这些菌株之间具有遗传相似性和代谢能力。这些菌株还表现出出色的促进植物生长和拮抗潜力，为全球粮食危机提供了潜在解决方案。本研究增进了我们对咸海西部盐碱地盐生植物中微生物多样性的理解，揭示了盐生植物微生物组及其在高盐环境中与宿主的协作关系。本研究还为开发高质量微生物肥料和实施可持续农业实践提供了科学依据。
