Nascimento Felipe Franco, Passareli João Victor Goulart Consoni, Zulim Luís Felipe da Costa, Silva Danielle Alves, Giuffrida Rogerio, Estanho Giovana José Garcia, Villa Mayra Claudino, Andrade Silvia Franco
Program of Animal Science, Universidade do Oeste Paulista, Presidente Prudente, SP, Brazil.
Faculty of Veterinary Medicine, Presidente Prudente, SP, Brazil.
Arq Bras Oftalmol. 2023 Jul-Aug;86(4):314-321. doi: 10.5935/0004-2749.20230057.
To compare strip meniscometry and Schirmer tear test 1 results and tear film breakup time between dogs with normal eyes and dogs diagnosed with keratoconjunctivitis sicca. Methods: One-hundred fifty-six eyes of 78 dogs, 88 normal eyes, and 68 eyes diagnosed with keratoconjunctivitis sicca were included in the study. The tests were performed in the following sequence: Schirmer tear test 1 was used to allocate the dogs to the normal or keratoconjunctivitis sicca group, followed by the strip meniscometry test and tear film breakup time measurement.
The results (mean ± standard deviation) of the tests in the normal group were as follows: Schirmer tear test 1, 22.75 ± 3.88 mm/min; strip meniscometry test, 10.01 ± 2.35 mm/5 sec; and tear film breakup time, 25.82 ± 5.47 sec. In the keratoconjunctivitis sicca group, the results were as follows: Schirmer tear test 1, 6.10 ± 4.44 mm/min; strip meniscometry test, 3.03 ± 2.62 mm/5 sec; and tear film breakup time, 10.78 ± 4.23 sec. The Spearman correlation coefficient in the keratoconjunctivitis sicca group was very high, with a significant difference between the strip meniscometry test and Schirmer tear test 1 (r=0.848, p<0.001), and moderate and significant between the strip meniscometry test and tear film breakup time (r=0.773, p<0.001). The cutoff for the strip meniscometry test for keratoconjunctivitis sicca was 7.0 mm/5 sec. Keratoconjunctivitis sicca was suspected when the values were <10 mm/5 sec.
This study provided strip meniscometry test values from dogs with normal eyes and eyes with keratoconjunctivitis sicca, with high sensitivity and specificity compared with those of the Schirmer tear test 1. In the future, the strip meniscometry test may be another important quantitative test and could complement the gold standard Schirmer tear test for the diagnosis of keratoconjunctivitis sicca in dogs, an excellent animal model for the study of the disease.
比较正常眼犬和被诊断为干眼症的犬的试纸条泪液半月板测量法结果、Schirmer泪液试验1结果及泪膜破裂时间。方法：本研究纳入了78只犬的156只眼，其中88只正常眼，68只被诊断为干眼症的眼。测试按以下顺序进行：使用Schirmer泪液试验1将犬分为正常组或干眼症组，随后进行试纸条泪液半月板测量法测试及泪膜破裂时间测量。
正常组测试结果（均值±标准差）如下：Schirmer泪液试验1为22.75±3.88毫米/分钟；试纸条泪液半月板测量法测试为10.01±2.35毫米/5秒；泪膜破裂时间为25.82±5.47秒。在干眼症组，结果如下：Schirmer泪液试验1为6.10±4.44毫米/分钟；试纸条泪液半月板测量法测试为3.03±2.62毫米/5秒；泪膜破裂时间为10.78±4.23秒。干眼症组的Spearman相关系数非常高，试纸条泪液半月板测量法测试与Schirmer泪液试验1之间存在显著差异（r=0.848，p<0.001），试纸条泪液半月板测量法测试与泪膜破裂时间之间存在中度且显著的差异（r=0.773，p<0.001）。干眼症试纸条泪液半月板测量法测试的临界值为7.0毫米/5秒。当数值<10毫米/5秒时怀疑为干眼症。
本研究提供了正常眼犬和干眼症犬的试纸条泪液半月板测量法测试值，与Schirmer泪液试验1相比具有高敏感性和特异性。未来，试纸条泪液半月板测量法测试可能是另一项重要的定量测试，可补充用于诊断犬干眼症的金标准Schirmer泪液试验，犬是研究该疾病的优秀动物模型。
