Department of Ophthalmology, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang, 325000, China.
Burn & Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Regen Med. 2022 Jun;17(6):375-388. doi: 10.2217/rme-2021-0173. Epub 2022 May 12.
This study aimed to explore the role of hypoxic mesenchymal stem cells (MSCs) in corneal alkali burns and the underlying mechanism. Rat corneal fibroblasts were incubated with IL-6, followed by treatment with hypoxic MSC supernatant. A rat corneal alkali burn model was implemented and processed with hypoxic MSCs. The associated factors were detected by corresponding methods. Hypoxic MSCs reduced the Notch1 level and the proliferation of rat corneal fibroblasts. Hypoxic MSCs or WWP2 overexpression in MSCs enhanced ubiquitination of Notch1. WWP2 interacted with Notch1, and WWP2 silencing reversed the effects of the hypoxic MSCs. Hypoxic MSC treatment decreased the corneal neovascularization scores and opacity scores. Hypoxic MSCs inhibited inflammation and alleviated corneal injury in alkali burns via the WWP2/Notch1 axis.
本研究旨在探讨低氧间充质干细胞（MSCs）在角膜碱烧伤中的作用及其机制。将大鼠角膜成纤维细胞与白细胞介素 6（IL-6）孵育，然后用低氧 MSC 上清液处理。建立大鼠角膜碱烧伤模型，并对低氧 MSC 进行处理。采用相应方法检测相关因子。低氧 MSC 降低了 Notch1 水平和大鼠角膜成纤维细胞的增殖。低氧 MSC 或 MSC 中 WWP2 的过表达增强了 Notch1 的泛素化。WWP2 与 Notch1 相互作用，WWP2 沉默逆转了低氧 MSC 的作用。低氧 MSC 处理降低了角膜新生血管评分和混浊评分。低氧 MSC 通过 WWP2/Notch1 轴抑制炎症反应，减轻碱烧伤引起的角膜损伤。
