Department of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, Germany.
Department of Gynecology and Gynecological Oncology, University Hospital Bonn, 53127 Bonn, Germany.
Cells. 2022 Sep 2;11(17):2740. doi: 10.3390/cells11172740.
Gingival wound healing plays an important role in the treatment of a variety of inflammatory diseases. In some cases, however, wound healing is delayed by various endogenous or exogenous factors. In recent years, non-invasive physical plasma (NIPP), a highly reactive gas, has become the focus of research, because of its anti-inflammatory and wound healing-promoting efficacy. So far, since NIPP application has been poorly elucidated in dentistry, the aim of this study was to further investigate the effect of NIPP on various molecules associated with inflammation and wound healing in gingival cells. Human gingival fibroblasts (HGF) and human gingival keratinocytes (HGK) were treated with NIPP at different application times. Cell viability and cell morphology were assessed using DAPI/phalloidin staining. Cyclooxygenase (COX)2; tumour necrosis factor (TNF); CC Motif Chemokine Ligand (CCL)2; and interleukin (IL)1B, IL6 and IL8 were analysed at the mRNA and protein level by a real-time PCR and ELISA. NIPP did not cause any damage to the cells. Furthermore, NIPP led to a downregulation of proinflammatory molecules. Our study shows that NIPP application does not damage the gingival tissue and that the promotion of wound healing is also due to an anti-inflammatory component.
牙龈伤口愈合在治疗各种炎症性疾病中起着重要作用。然而，在某些情况下，由于各种内源性或外源性因素，伤口愈合会延迟。近年来，非侵入性物理等离子体（NIPP）作为一种高反应性气体，因其具有抗炎和促进伤口愈合的功效而成为研究的焦点。到目前为止，由于 NIPP 在牙科中的应用尚未得到充分阐明，本研究旨在进一步研究 NIPP 对牙龈细胞中与炎症和伤口愈合相关的各种分子的影响。用 NIPP 处理不同作用时间的人牙龈成纤维细胞（HGF）和人牙龈角化细胞（HGK）。用 DAPI/鬼笔环肽染色评估细胞活力和细胞形态。通过实时 PCR 和 ELISA 分析环氧化酶（COX）2；肿瘤坏死因子（TNF）；CC 基序趋化因子配体（CCL）2；白细胞介素（IL）1B、IL6 和 IL8 的 mRNA 和蛋白水平。NIPP 不会对细胞造成任何损伤。此外，NIPP 导致促炎分子的下调。我们的研究表明，NIPP 应用不会损伤牙龈组织，促进伤口愈合也归因于其抗炎成分。
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