Life and Health Intelligent Research Institute, Tianjin University of Technology, Tianjin, 300384, P. R. China.
Department of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, P. R. China.
Angew Chem Int Ed Engl. 2022 Jul 18;61(29):e202205628. doi: 10.1002/anie.202205628. Epub 2022 Jun 1.
The detection of biomarkers in tears has aroused great interest owing to the advantages of non-invasive and rapid collection. The combination of ultrasensitivity and label-free detection of surface-enhanced Raman spectroscopy (SERS) sensors is expected to achieve real-time diagnosis in home medical care. However, the surface of SERS sensors is susceptible to biofouling and inactivation by biological impurities in tears, resulting in rapid degradation of sensitivity, limiting the commercialization of point-of-care devices. Herein, a binary nanosphere array with dual properties is constructed as a separation-sensing platform for the diagnosis of target molecules in tears. The upper part of the structure is composed of Au nanoparticles (AuNPs) and a sputtering Au layer, which can bind the target molecules that interact with Au and provide high-strength and high-density SERS hotspots. The lower half is an inactive SiO nanosphere array with periodic large pores that allows biological impurities to penetrate the lower part and be separated from the target analyte. Furthermore, this substrate was integrated into homemade tear kits, enabling simultaneous tear collection, pre-separation, and detection. Combined with the Raman spectra of tears and LDA analysis, we successfully identified patients with jaundice in clinics. This platform is expected to provide an opportunity for early disease screening based on biological fluids.
由于非侵入性和快速采集的优势，泪液中生物标志物的检测引起了极大的兴趣。表面增强拉曼光谱（SERS）传感器的超灵敏性和无标记检测的结合有望实现家庭医疗的实时诊断。然而，SERS 传感器的表面容易受到生物杂质的生物污垢和失活，导致灵敏度迅速下降，限制了即时护理设备的商业化。在此，构建了一种具有双重特性的二元纳米球阵列作为分离传感平台，用于诊断泪液中的靶分子。结构的上半部分由金纳米颗粒（AuNPs）和溅射 Au 层组成，可与 Au 相互作用的靶分子结合，并提供高强度和高密度的 SERS 热点。下半部分是具有周期性大孔的非活性 SiO2 纳米球阵列，允许生物杂质穿透下部并与目标分析物分离。此外，该基底被集成到自制的泪液试剂盒中，能够同时进行泪液采集、预分离和检测。结合泪液的拉曼光谱和 LDA 分析，我们成功地在临床上识别出黄疸患者。该平台有望为基于生物流体的早期疾病筛查提供机会。
