Department of Biological Sciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore, MP, 453331, India.
Centre for Wildlife Health, College of Veterinary Sciences and Animal Husbandry, Bhubaneswar, 751030, India.
Arch Virol. 2022 Jul;167(7):1571-1576. doi: 10.1007/s00705-022-05443-5. Epub 2022 May 11.
Contagious pustular dermatitis is a disease that primarily infects small ruminants and possesses zoonotic potential. It is caused by orf virus (ORFV), a member of the genus Parapoxvirus. In this study, we evaluated an ORFV outbreak in goats in Madhya Pradesh, a state in central India, during 2017. The transboundary potential of this virus was evaluated by constructing phylogenetic trees. The complete genome sequence of an ORFV isolate named Ind/MP/17 was found to be 139,807 bp in length with 63.7% GC content and 132 open reading frames (ORFs) flanked by 3,910-bp inverted terminal repeats (ITRs). An investigation into evolutionary parameters such as selection pressure (θ = dN/dS) and nucleotide diversity (π) demonstrated that ORFV has undergone purifying selection. A total of 40 recombination events were identified, 21 of which were evident in the Ind/MP/17 genome, indicating its ability to generate new variants.
接触传染性脓疱性皮炎是一种主要感染小反刍动物的疾病，具有潜在的人畜共患病性。它是由口疮病毒（ORFV）引起的，口疮病毒是副痘病毒属的一员。在这项研究中，我们评估了印度中央邦 2017 年山羊中的一次 ORFV 爆发。通过构建系统发育树评估了该病毒的跨界潜力。命名为 Ind/MP/17 的 ORFV 分离株的完整基因组序列长 139807bp，GC 含量为 63.7%，由 3910bp 的反向末端重复序列（ITR）侧翼，有 132 个开放阅读框（ORF）。对选择压力（θ=dN/dS）和核苷酸多样性（π）等进化参数的调查表明，ORFV 经历了纯化选择。共鉴定出 40 个重组事件，其中 21 个在 Ind/MP/17 基因组中明显，表明其具有产生新变体的能力。
