Biological Effects of Aqueous Extract of L. Leaves on Some Histological and Immunological Parameters in Male Rat Liver Affected by Aluminum Chloride.

The present current study aimed to assess the protective effect of the aqueous extract of L. leaves against the toxic effects of aluminum chloride on liver tissue. A number of 36 male albino rats (Wistar) were randomly assigned to six groups (n=6) and treated for 30 days: Group 1 was regarded as the control group, Group 2 received Aluminum Chloride 90 mg/kg body weight orally by gavage, Group3: normal rats received aqueous extracts of L. leaf 150 mg/kg body weight, Group 4: normal rats received aqueous extracts of L. leaf 200 mg/kg body weight, Group 5: normal rats received aqueous extracts of L. leaf 150 mg/kg body weight after a period of 4 h following treatment by Aluminum Chloride 90 mg/kg body weight, Group 6: normal rats received aqueous extracts of L. 200 mg /kg after a period of 4 h following treatment by Aluminum chloride with 90 mg/kg body weight. All the experimental animals were sacrificed, and sections of their liver were stained with Hematoxylin-Eosin for histological evaluations. Moreover, the liver enzymes and immune cytokines, such as Alkaline phosphatase (ALP), Alanine aminotransferase (ALT), and Aspartate aminotransferase (AST), tumor necrosis factor-alpha (TNF-alpha), and interleukin-10 (IL-10) were measured. As evidenced by the results of the current study, treatment with aqueous extract of L. leaves at a dose of 150 and 200 mg/kg body weight orally contributed to the mitigation of the toxic effects of Aluminum Chloride in albino rats by reducing the damage and inflammation in the hepatocytes. The study suggested that the aqueous extract of L. enhances the protective effect against liver toxicity.