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一种新型荧光长链脂肪酸取代染料:对……的标记与生物降解

A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of .

作者信息

Gu Yingchun, Lin Dayong, Fei Xuening, Wang Cuihong, Yang Qi, Tang Yalin, Ren Xueling

机构信息

Tianjin Engineering Technology Center of Chemical Wastewater Source Reduction and Recycling, School of Science, Tianjin Chengjian University Tianjin 300384 China

Anda Duosen New Material Technology CO., LTD Heilongjiang Province 151400 China.

出版信息

RSC Adv. 2018 Oct 22;8(62):35855-35862. doi: 10.1039/c8ra06986g. eCollection 2018 Oct 15.

Abstract

() is a filamentous bacterium that induces bulking in activated sludge. Here, we used the affinity of long-chain fatty acids (LCFA) for to create a novel fluorescent probe of carbazole modified by LCFA. The structure was characterized by H NMR spectroscopy and mass spectrometry. The spectral properties, photostability, and hydrophobic properties of the probe were also characterized. Fluorescent-labeling results showed that it can label and could be biodegraded metabolism. The stable docking mode of carbazole probes with different fatty acid chains and lipases was also docked by the density functional tight-binding (DFTB) method.

摘要

()是一种导致活性污泥膨胀的丝状细菌。在此,我们利用长链脂肪酸(LCFA)对(此处原文有缺失信息)的亲和力,制备了一种由LCFA修饰的咔唑新型荧光探针。通过核磁共振氢谱(H NMR)光谱和质谱对其结构进行了表征。还对该探针的光谱特性、光稳定性和疏水特性进行了表征。荧光标记结果表明,它可以标记(此处原文有缺失信息)并且可以通过代谢被生物降解。还通过密度泛函紧束缚(DFTB)方法对接了咔唑探针与不同脂肪酸链和脂肪酶的稳定对接模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/272f/9088196/8dc301525940/c8ra06986g-f8.jpg

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