用于原位鉴定细小丝硫菌的新型荧光探针的设计机制与性能
Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ.
作者信息
Jiao Xiumei, Fei Xuening, Li Songya, Lin Dayong, Ma Huaji, Zhang Baolian
机构信息
School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China.
出版信息
Materials (Basel). 2017 Jul 15;10(7):804. doi: 10.3390/ma10070804.
In this study, two novel fluorescent probes, probe A and probe B were designed, synthesized and characterized, based on () preferring to utilize long-chain fatty acid (LCFA), for the labeling of in activated sludge. The molecular structure of probe A and probe B include long-chain alkane and LCFA, respectively. The results indicated that probe A and probe B had a large stokes shift of 118 nm and 120 nm and high quantum yield of 0.1043 and 0.1058, respectively, which were significantly helpful for the fluorescent labeling. As probe A was more stable than probe B in activated sludge, and the fluorescence intensity keep stable during 24 h, probe A was more suitable for labeling in situ. In addition, through the Image Pro Plus 6 (IPP 6) analysis, a quantitative relationship was established between sludge volume index (SVI) and integral optical density (IOD) of the labeled in activated sludge samples. The relationship between IOD and SVI conforms to Logistic curve (² = 0.94).
在本研究中，基于（）倾向于利用长链脂肪酸（LCFA），设计、合成并表征了两种新型荧光探针，探针A和探针B，用于标记活性污泥中的（）。探针A和探针B的分子结构分别包括长链烷烃和LCFA。结果表明，探针A和探针B的斯托克斯位移分别为118 nm和120 nm，量子产率分别为0.1043和0.1058，这对荧光标记有显著帮助。由于探针A在活性污泥中比探针B更稳定，且荧光强度在24小时内保持稳定，探针A更适合原位标记（）。此外，通过Image Pro Plus 6（IPP 6）分析，建立了活性污泥样品中污泥体积指数（SVI）与标记的（）的积分光密度（IOD）之间的定量关系。IOD与SVI之间的关系符合逻辑曲线（² = 0.94）。
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