Liu Hailong, Lian Bin
College of Life Sciences, Nanjing Normal University No. 1, WenYuan Road, QiXia District Nanjing 210023 Jiangsu Province China
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences Guiyang China.
RSC Adv. 2018 Aug 20;8(51):29476-29481. doi: 10.1039/c8ra05006f. eCollection 2018 Aug 14.
TiO-based MOAC (metal oxide affinity chromatography) nanomaterials are regarded as one of the most promising materials for phosphopeptide enrichment. However, the serious non-specific adsorption of acidic peptides and the limited chemisorption performance to phosphopeptides will greatly reduce the enrichment efficiency. To overcome the above problems, a novel TiO hybrid material with guanidyl-functionalized TiO nanoparticles (GF-TiO) anchored on the surface of a graphene oxide (GO) platform (denoted as GF-TiO-GO) is successfully synthesized and applied as a biofunctional adsorbent for selective enrichment of trace phosphopeptides. Due to the improved selectivity to phosphopeptides and larger loading capacity, the novel GF-TiO-GO nanohybrids exhibited higher selectivity toward phosphopeptides and a lower detection limit even when the concentration of β-casein was decreased to only 1 × 10 M. The selective enrichment test toward phosphopeptides from the tryptic digests of nonfat milk and human serum further validated that the GF-TiO-GO nanohybrids were capable of selectively capturing global phosphopeptides from complicated biological samples.
基于二氧化钛的金属氧化物亲和色谱（MOAC）纳米材料被认为是用于磷酸化肽富集的最有前途的材料之一。然而，酸性肽严重的非特异性吸附以及对磷酸化肽有限的化学吸附性能会大大降低富集效率。为了克服上述问题，一种新型的二氧化钛杂化材料成功合成，该材料将胍基功能化的二氧化钛纳米颗粒（GF-TiO）锚定在氧化石墨烯（GO）平台表面（记为GF-TiO-GO），并用作生物功能吸附剂以选择性富集痕量磷酸化肽。由于对磷酸化肽的选择性提高和更大的负载能力，即使β-酪蛋白浓度降至仅1×10⁻⁷ M，新型GF-TiO-GO纳米杂化物对磷酸化肽仍表现出更高的选择性和更低的检测限。对脱脂牛奶和人血清胰蛋白酶消化物中磷酸化肽的选择性富集测试进一步验证了GF-TiO-GO纳米杂化物能够从复杂生物样品中选择性捕获整体磷酸化肽。
