Mo Yuxing, Jiang Bian, Huo Jingxin, Lu Jiayi, Zeng Xiaoyue, Zhou Yan, Zhang Tao, Yang Min, Wei Yuerong, Liu Kaidong
Life Science and Technology School, Lingnan Normal University, Zhanjiang, China.
Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MOA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Front Plant Sci. 2022 Apr 25;13:890581. doi: 10.3389/fpls.2022.890581. eCollection 2022.
Lysine ubiquitination is a highly conserved post-translational modification with diverse biological functions. However, there is little available information on lysine ubiquitination of non-histone proteins in papaya ( L.). In total, 3,090 ubiquitination sites on 1,249 proteins with diverse localizations and functions were identified. Five conserved ubiquitinated K motifs were identified. Enrichment analysis showed that many Hsps were differentially ubiquitinated proteins (DUPs), suggesting an essential role of ubiquitination in degradation of molecular chaperone. Furthermore, 12 sugar metabolism-related enzymes were identified as DUPs, including an involvement of ubiquitination in nutrimental changes during the papaya ripening process. The ubiquitination levels of five fruit ripening-related DUPs, including one ethylene-inducible protein, two 1-aminocyclopropane-1-carboxylic acid oxidases, one endochitinase, and one cell wall invertase, were significantly changed during the ripening process. Our study extends the understanding of diverse functions for lysine ubiquitination in regulation of the papaya fruit ripening process.
赖氨酸泛素化是一种高度保守的翻译后修饰，具有多种生物学功能。然而，关于番木瓜（L.）中非组蛋白蛋白质的赖氨酸泛素化的可用信息很少。总共鉴定出1249种具有不同定位和功能的蛋白质上的3090个泛素化位点。鉴定出五个保守的泛素化K基序。富集分析表明，许多热休克蛋白是差异泛素化蛋白（DUPs），表明泛素化在分子伴侣降解中起重要作用。此外，鉴定出12种与糖代谢相关的酶为DUPs，包括泛素化参与番木瓜成熟过程中的营养变化。包括一种乙烯诱导蛋白、两种1-氨基环丙烷-1-羧酸氧化酶、一种内切几丁质酶和一种细胞壁转化酶在内的五种与果实成熟相关的DUPs的泛素化水平在成熟过程中发生了显著变化。我们的研究扩展了对赖氨酸泛素化在调控番木瓜果实成熟过程中的多种功能的理解。
