Life Science and Technology School, Lingnan Normal University, Zhanjiang, Guangdong, 524048, China.
College of Bioscience and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Sci Rep. 2018 May 29;8(1):8230. doi: 10.1038/s41598-018-26676-y.
Lysine crotonylation of histone proteins is a recently-identified post-translational modification with multiple cellular functions. However, no information about lysine crotonylation of non-histone proteins in fruit cells is available. Using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity antibody analysis, a global crotonylation proteome analysis of papaya fruit (Carica papaya L.) was performed. In total, 2,120 proteins with 5,995 lysine crotonylation sites were discovered, among which eight conserved motifs were identified. Bioinformatic analysis linked crotonylated proteins to multiple metabolic pathways, including biosynthesis of antibiotics, carbon metabolism, biosynthesis of amino acids, and glycolysis. particularly, 40 crotonylated enzymes involved in various pathways of amino acid metabolism were identified, suggesting a potential conserved function for crotonylation in the regulation of amino acid metabolism. Numerous crotonylation sites were identified in proteins involved in the hormone signaling and cell wall-related pathways. Our comprehensive crotonylation proteome indicated diverse functions for lysine crotonylation in papaya.
组蛋白赖氨酸的琥珀酰化是一种新发现的具有多种细胞功能的翻译后修饰。然而，关于果实细胞中非组蛋白的赖氨酸琥珀酰化还没有信息。本研究采用高分辨率 LC-MS/MS 结合高灵敏度的免疫亲和抗体分析方法，对番木瓜果实（Carica papaya L.）进行了全琥珀酰化蛋白质组分析。共发现了 2120 种蛋白质，其中包含 5995 个赖氨酸琥珀酰化位点，鉴定出 8 个保守基序。生物信息学分析将琥珀酰化蛋白与多种代谢途径联系起来，包括抗生素的生物合成、碳代谢、氨基酸的生物合成和糖酵解。特别是，鉴定出了 40 种参与氨基酸代谢不同途径的琥珀酰化酶，表明琥珀酰化在调节氨基酸代谢中的潜在保守功能。在参与激素信号和细胞壁相关途径的蛋白质中也鉴定到了许多琥珀酰化位点。本研究的全面琥珀酰化蛋白质组表明，赖氨酸琥珀酰化在番木瓜中有多种功能。
