Zhao Zhi, Li Meng, Zhang He, Yu Yao, Ma Lu, Wang Wei, Fan Yunxin, Huang Ning, Wang Xinying, Liu Kunquan, Dong Shinan, Tang Haijuan, Wang Jianfei, Zhang Hongsheng, Bao Yongmei
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Jiangsu Collaborative Innovation Center for Modern Crop Production, Cyrus Tang Innovation Center for Crop Seed Industry, Jiangsu Province Engineering Research Center of Seed Industry Science and Technology, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2022 Apr 25;13:853195. doi: 10.3389/fpls.2022.853195. eCollection 2022.
Rice blast, caused by , is one of the most devastating diseases in rice and can affect rice production worldwide. Rice plasma membrane (PM) proteins are crucial for rapidly and precisely establishing a defense response in plant immunity when rice and blast fungi interact. However, the plant-immunity-associated vesicle trafficking network mediated by PM proteins is poorly understood. In this study, to explore changes in PM proteins during infection, the PM proteome was analyzed iTRAQ in the resistant rice landrace Heikezijing. A total of 831 differentially expressed proteins (DEPs) were identified, including 434 upregulated and 397 downregulated DEPs. In functional analyses, DEPs associated with vesicle trafficking were significantly enriched, including the "transport" term in a Gene Ontology enrichment analysis, the endocytosis and phagosome pathways in a Encyclopedia of Genes and Genomes analysis, and vesicle-associated proteins identified a protein-protein interaction network analysis. OsNPSN13, a novel plant-specific soluble -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) 13 protein, was identified as an upregulated DEP, and transgenic plants overexpressing this gene showed enhanced blast resistance, while transgenic knockdown plants were more susceptible than wild-type plants. The changes in abundance and putative functions of 20 DEPs revealed a possible vesicle trafficking network in the -rice interaction. A comparative proteomic analysis of plasma membrane proteins in rice leaves revealed a plant-immunity-associated vesicle trafficking network that is provoked by blast fungi; these results provide new insights into rice resistance responses against rice blast fungi.
稻瘟病由[病原体名称缺失]引起，是水稻中最具毁灭性的病害之一，可影响全球水稻产量。当水稻与稻瘟病菌相互作用时，水稻质膜（PM）蛋白对于在植物免疫中快速准确地建立防御反应至关重要。然而，由PM蛋白介导的与植物免疫相关的囊泡运输网络目前了解甚少。在本研究中，为了探究感染过程中PM蛋白的变化，利用iTRAQ技术对抗性水稻地方品种黑壳子粳的PM蛋白质组进行了分析。共鉴定出831个差异表达蛋白（DEP），其中434个上调，397个下调。在功能分析中，与囊泡运输相关的DEP显著富集，包括基因本体富集分析中的“运输”术语、基因与基因组百科全书分析中的内吞作用和吞噬体途径，以及在蛋白质-蛋白质相互作用网络分析中鉴定出的囊泡相关蛋白。OsNPSN13是一种新型的植物特异性可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）13蛋白，被鉴定为上调的DEP，过表达该基因的转基因植物表现出增强的稻瘟病抗性，而转基因敲低植物比野生型植物更易感病。20个DEP的丰度和假定功能变化揭示了稻瘟病菌与水稻相互作用中可能存在的囊泡运输网络。对水稻叶片质膜蛋白的比较蛋白质组学分析揭示了一种由稻瘟病菌引发的与植物免疫相关的囊泡运输网络；这些结果为水稻对稻瘟病菌的抗性反应提供了新的见解。
