Li Wen Qi, Jia Yu Lin, Liu Feng Quan, Wang Fang Quan, Fan Fang Jun, Wang Jun, Zhu Jin Yan, Xu Yang, Zhong Wei Gong, Yang Jie
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing Branch of Chinese National Center for Rice Improvement, Jiangsu High Quality Rice R&D Center Nanjing 210014 China
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University Yangzhou 225009 China.
RSC Adv. 2018 Oct 8;8(60):34408-34417. doi: 10.1039/c8ra04993a. eCollection 2018 Oct 4.
Plant long non-coding RNA (lncRNA) is a type of newly emerging epigenetic regulator playing a critical role in plant growth, development, and biotic stress responses. However, it is unknown whether lncRNAs are involved in resistance responses between rice and , a bacterial agent causing rice foot rot disease. In this study, RNA-seq was performed to uncover the co-expression regulating networks mediated by responsive lncRNAs and their candidate target genes. Of the 4709 lncRNAs identified, 2518 and 2191 were up- and down-regulated in response to infection, respectively. Expression changes of 17 selected lncRNAs and their predicted targets with a potential role in defense response were investigated by qPCR. The expression levels of five lncRNAs were up-regulated while their cognate candidate target genes were down-regulated upon infection. In addition, several lncRNAs were predicted to be target mimics of osa-miR396 and osa-miR156. These results suggest that lncRNAs might play a role in response to infection by regulating the transcript levels of their targets and miRNAs in rice.
植物长链非编码RNA（lncRNA）是一类新出现的表观遗传调控因子，在植物生长、发育及生物胁迫响应中发挥关键作用。然而，尚不清楚lncRNAs是否参与水稻与引起水稻基腐病的细菌病原体之间的抗性反应。在本研究中，进行了RNA测序以揭示由响应性lncRNAs及其候选靶基因介导的共表达调控网络。在鉴定出的4709个lncRNAs中，分别有2518个和2191个在响应病原体感染时上调和下调。通过qPCR研究了17个选定的lncRNAs及其预测的在防御反应中具有潜在作用的靶标的表达变化。在病原体感染时，5个lncRNAs的表达水平上调，而其同源候选靶基因的表达水平下调。此外，预测有几个lncRNAs是osa-miR396和osa-miR156的靶标模拟物。这些结果表明，lncRNAs可能通过调节其靶标和miRNAs在水稻中的转录水平，在响应病原体感染中发挥作用。
