Dai Huang, Li Yuqing, Zhang Qi, Fu Yingchun, Li Yanbin
College of Biosystems Engineering and Food Science, Zhejiang University Hangzhou 310058 China
Department of Biological and Agricultural Engineering, University of Arkansas Fayetteville AR 72701 USA.
RSC Adv. 2018 Oct 3;8(59):33960-33967. doi: 10.1039/c8ra06347h. eCollection 2018 Sep 28.
Sensitive and selective colorimetric sensors have come into a high demand due to their simplicity, rapidity, precision and use of common laboratory instruments. In this study, as a new colorimetric nanoprobe, enzyme-catalysis-induced production of Prussian blue nanoparticles (PBNPs) was employed to develop a colorimetric biosensor which was simple and inexpensive for the rapid detection of glucose in wine. Briefly, glucose as the detection target was added into a solution of glucose oxidase (GOx), FeCl and KFe(CN), which turned the solution color from light-yellow to blue within 10 min. Thus, it could be probed by UV-vis spectroscopy. Unlike common colorimetric methods based on a sole color change mechanism, this method has two paths to generate PBNPs. Because both KFe(CN) and O are involved in the turnover of GOx catalysis, they generate KFe(CN) and HO that reduces Fe, respectively, and both paths finally produce PBNPs. This dual-path method enhances the yield of PBNPs and the detection performance. Under optimized conditions, the method presented a linear detection range of 4 μM to 0.5 mM ( = 0.998) and a limit of detection of 3.29 μM, which is comparable to or better than analogues, as well as excellent selectivity. This method also worked well in white grape wine samples with detection results varying within 1% to those obtained by the standard HPLC method. The proposed biosensing method is rapid, simple, low-cost, sensitive and selective, therefore, it is a promising method for daily detection of glucose in food in households and markets.
由于其操作简单、检测快速、结果精确且使用常见实验室仪器，灵敏且具选择性的比色传感器需求大增。在本研究中，作为一种新型比色纳米探针，利用酶催化诱导生成普鲁士蓝纳米颗粒（PBNPs）来开发一种比色生物传感器，该传感器用于快速检测葡萄酒中的葡萄糖，具有简单且成本低的特点。简而言之，将作为检测目标的葡萄糖加入葡萄糖氧化酶（GOx）、FeCl和KFe(CN)的溶液中，溶液在10分钟内由浅黄色变为蓝色。因此，可通过紫外可见光谱进行检测。与基于单一颜色变化机制的普通比色法不同，该方法有两条生成PBNPs的途径。由于KFe(CN)和O都参与GOx催化的周转，它们分别生成KFe(CN)和还原Fe的HO，两条途径最终都产生PBNPs。这种双途径方法提高了PBNPs的产量和检测性能。在优化条件下，该方法的线性检测范围为4 μM至0.5 mM（ = 0.998），检测限为3.29 μM，与类似方法相当或更优，且具有出色的选择性。该方法在白葡萄酒样品中也表现良好，检测结果与标准HPLC方法的结果相差在1%以内。所提出的生物传感方法快速、简单、低成本、灵敏且具选择性，因此，它是家庭和市场中日常检测食品中葡萄糖的一种有前景的方法。
