一种基于倍半硅氧烷（POSS）的荧光探针，用于促进活细胞和斑马鱼中次氯酸（HClO）和一氧化氮（NO）的可视化。
A fluorescent probe based on POSS for facilitating the visualization of HClO and NO in living cells and zebrafish.
作者信息
Ding Guowei, Gai Fengqing, Gou Zhiming, Zuo Yujing
机构信息
School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, P. R. China.
出版信息
Anal Methods. 2022 May 27;14(20):2035-2042. doi: 10.1039/d2ay00482h.
The main production area of HClO and NO is the mitochondria and has modulatory effects on multiple human diseases. Simultaneous detection of signaling molecules such as HClO and NO is an important approach for exploring the complex relationship between HClO and NO in mitochondria. However, most probes can detect only one species or are unable to complete the monitoring of HClO and NO in the NIR channel. There are only few reports on reasonable tools that can simultaneously monitor the presence of HClO and NO in the NIR channel. In this work, to solve this difficulty, a POSS-assisted NIR fluorescent probe with dual-response was rationally devised and developed. The probe Mito-Cy possessed high specificity and responsiveness to HClO and NO in spectral experiments. Notably, the probe exhibited excellent responsiveness and sensitivity to HClO and NO in living cells and the zebrafish model.
次氯酸（HClO）和一氧化氮（NO）的主要产生部位是线粒体，且对多种人类疾病具有调节作用。同时检测HClO和NO等信号分子是探索线粒体中HClO与NO复杂关系的重要途径。然而，大多数探针只能检测一种物质，或者无法在近红外（NIR）通道完成对HClO和NO的监测。关于能够在NIR通道同时监测HClO和NO存在的合理工具的报道很少。在这项工作中，为了解决这一难题，合理设计并开发了一种具有双响应的POSS辅助近红外荧光探针。在光谱实验中，探针Mito-Cy对HClO和NO具有高特异性和响应性。值得注意的是，该探针在活细胞和斑马鱼模型中对HClO和NO表现出优异的响应性和灵敏度。
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