Lai-Goldman M, McBride J H, Howanitz P J, Rodgerson D O, Miles J A, Peter J B
Am J Clin Pathol. 1987 May;87(5):635-9. doi: 10.1093/ajcp/87.5.635.
The authors tested 15 immune serum globulin pharmaceutical preparations for antibody reactivity to human T cell lymphotrophic virus type III (HTLV-III) by the Abbott immunoenzymometric assay (IEMA). Although no evidence of HTLV-III infectivity has appeared after injection of similar preparations into humans, the authors found all samples IEMA reactive. Upon dilution, the authors demonstrated parallel decreases of antibody reactivity among two samples of gamma globulin, the Abbott-positive control, and a markedly reactive patient specimen. Gamma globulin isolated from sera of six animal species was nonreactive in the Abbott assay. All samples were nonreactive with the H-9 cell line antigen. Antibody reactivity to HTLV-III was confirmed in 13 of 15 gamma globulin samples when tested by the Electro-Nucleonics IEMA, and 14 samples contained at least the p24 band on Western blot analysis. Although false positivity occurs in IEMA assays possibly because of elevated protein concentrations and nonspecific binding, the authors, results show that in most circumstances immune serum globulin preparations tested do contain true reactivity to HTLV-III.
作者们通过雅培免疫酶量测定法（IEMA）检测了15种免疫血清球蛋白药物制剂对人类III型嗜T细胞病毒（HTLV - III）的抗体反应性。尽管向人体注射类似制剂后未出现HTLV - III感染的证据，但作者们发现所有样本在IEMA检测中均呈反应性。稀释后，作者们证明了两种γ球蛋白样本、雅培阳性对照以及一份反应明显的患者标本中抗体反应性呈平行下降。从六种动物血清中分离出的γ球蛋白在雅培检测中无反应性。所有样本与H - 9细胞系抗原均无反应性。当通过电子核子学IEMA检测时，15份γ球蛋白样本中有13份对HTLV - III的抗体反应性得到确认，并且在蛋白质印迹分析中14份样本至少含有p24条带。尽管IEMA检测中可能由于蛋白质浓度升高和非特异性结合而出现假阳性，但作者们的结果表明，在大多数情况下，所检测的免疫血清球蛋白制剂确实对HTLV - III具有真实反应性。
