免疫血清球蛋白以及接受免疫血清球蛋白的人类受者血清中针对甲型肝炎病毒的中和抗体。

Neutralizing antibody to hepatitis A virus in immune serum globulin and in the sera of human recipients of immune serum globulin.

作者信息

Stapleton J T, Jansen R, Lemon S M

出版信息

Gastroenterology. 1985 Sep;89(3):637-42. doi: 10.1016/0016-5085(85)90462-7.

Abstract

We determined the titer of neutralizing antibody to hepatitis A virus in five lots of immune serum globulin and in the sera of human recipients of immune globulin using a new and sensitive procedure, the radioimmunofocus inhibition assay. The neutralizing antibody titer of four immune globulin lots ranged from 1:170,000 to 1:406,000, and was approximately 100-fold the antibody titer determined by radioimmunoassay. The neutralizing antibody titer of a Bureau of Biologics reference immunoglobulin was 1:794,000. Sera collected from 18 healthy men who had undergone prophylaxis with immune globulin (0.02 ml/kg body wt) were negative when tested by radioimmunoassay. However, 2 of 18 sera collected before administration of immune globulin and sera from all 18 men collected 3 and 55 days later contained detectable neutralizing antibody. The measurement of neutralizing antibody should prove useful for standardizing passive immunoprophylaxis against hepatitis A as well as for evaluating the potential efficacy of new hepatitis A virus vaccines.

摘要

我们采用一种新的灵敏方法——放射免疫聚焦抑制试验，测定了五批免疫血清球蛋白以及接受免疫球蛋白的人类受者血清中甲型肝炎病毒中和抗体的滴度。四批免疫球蛋白的中和抗体滴度在1:170,000至1:406,000之间，约为放射免疫测定法所测抗体滴度的100倍。生物制品局参考免疫球蛋白的中和抗体滴度为1:794,000。对18名接受免疫球蛋白（0.02 ml/kg体重）预防的健康男性采集的血清进行放射免疫测定时呈阴性。然而，在给予免疫球蛋白之前采集的18份血清中有2份，以及在3天和55天后采集的所有18名男性的血清中均含有可检测到的中和抗体。中和抗体的测定对于甲型肝炎被动免疫预防的标准化以及评估新型甲型肝炎病毒疫苗的潜在疗效应是有用的。