Enzyme Technology Laboratory, Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Enzyme Technology Laboratory, Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Bioelectrochemistry. 2022 Aug;146:108144. doi: 10.1016/j.bioelechem.2022.108144. Epub 2022 Apr 29.
Laccases have been in the spotlight due to its capability to catalyse electrocatalytic reactions. The Its ability to reduce water to oxygen has made it more bio-compatible than majority of electrocatalysts. The exploitation of such biocatalysts via protein engineering and biotechnological techniques have rendered a significant breakthrough in the field of electrocatalysis. This review aims to provide a comprehensive overview of the various laccase-driven electrocatalytic reactions. Structural and functional features of laccases that contribute towards the electron transfer mechanism and reduction potential have been meticulously described. Tailoring of laccase affords an excellent prospect for its application in diverse fields like, biocathode fabrication for enzymatic biofuel cells, biosensors, and electrocatalytic reactions like water splitting. The substantial knowledge of enzyme engineering, primarily through site directed mutagenesis is propitious for the design of optimal bioelectrocatalyst. This review seeks to provide an insight for the optimisation of electrocatalytic properties of laccase thereby broadening the horizon for the development of resilient and biocompatible bioelectrocatalyst.
漆酶因其催化电催化反应的能力而成为焦点。它将水还原为氧气的能力使其比大多数电催化剂更具生物相容性。通过蛋白质工程和生物技术技术对这些生物催化剂的开发，在电催化领域取得了重大突破。本综述旨在全面概述各种漆酶驱动的电催化反应。详细描述了漆酶的结构和功能特征，这些特征有助于电子转移机制和还原电位。对漆酶进行修饰为其在各种领域的应用提供了极好的前景，例如酶生物燃料电池的生物阴极制造、生物传感器以及电催化反应，如水分解。通过定点突变等主要方法对酶工程的深入了解有利于设计最佳的生物电催化剂。本综述旨在为优化漆酶的电催化性能提供深入了解，从而拓宽开发有弹性和生物相容性的生物电催化剂的视野。
