Mancini P, Fertels S, Nave D, Gealt M A
Appl Environ Microbiol. 1987 Apr;53(4):665-71. doi: 10.1128/aem.53.4.665-671.1987.
The mobilization of plasmid pHSV106 from Escherichia coli HB101, in a laboratory model waste treatment facility, by both laboratory and indigenous wastewater strains of E. coli was monitored by transfer of antibiotic resistance characteristics and by detection of pHSV106 DNA sequences in recipient cells. The mobilization was demonstrated to occur under several different treatment conditions, such as different media composition, microbial concentrations, and waste flow rates. The herpes simplex virus thymidine kinase gene was used as a hybridization marker to confirm the occurrence of the transfer. The use of the HB101 (recA mutant) host cell implies that recA functions are unnecessary for the gene transfer.
在实验室模型废水处理设施中，通过抗生素抗性特征的转移以及受体细胞中pHSV106 DNA序列的检测，监测了来自大肠杆菌HB101的质粒pHSV106被实验室菌株和本地废水大肠杆菌菌株的转移情况。结果表明，在几种不同的处理条件下，如不同的培养基组成、微生物浓度和废水流速下，转移均会发生。单纯疱疹病毒胸苷激酶基因用作杂交标记来确认转移的发生。使用HB101（recA突变体）宿主细胞意味着recA功能对于基因转移并非必需。
