Herman C J, McGraw T P, Marder R J, Bauer K D
Arch Pathol Lab Med. 1987 Jun;111(6):505-12.
The last several years have seen changes in quantitative cytologic procedures that have significantly expanded the clinical research and diagnostic applications of quantitative cytologic analysis. Intact nuclei can be isolated from formalin-fixed paraffin-embedded tissue, and the DNA distribution of these nuclei can be analyzed either with flow cytometry or with slide-based image-processing techniques. In addition, more informative DNA analysis has been achieved using antibodies to bromodeoxyuridine-modified DNA, proliferation-associated nuclear antigens, or tissue-type-specific cytoplasmic antigens as second stains for two-parameter analysis. Improvements in both staining and instrumentation have also increased the usefulness of flow cytometry in analysis of "rare events," ie, cell types comprising less than 0.1% to 0.5% of a total cell suspension. Commercially available slide-based image-processing systems have been developed that provide sophisticated cell and tissue analysis functions using inexpensive personal-computer-based systems.
在过去几年中，定量细胞学程序发生了变化，这极大地扩展了定量细胞学分析的临床研究和诊断应用。完整的细胞核可以从福尔马林固定石蜡包埋组织中分离出来，这些细胞核的DNA分布可以通过流式细胞术或基于玻片的图像处理技术进行分析。此外，使用针对溴脱氧尿苷修饰的DNA、增殖相关核抗原或组织类型特异性细胞质抗原的抗体作为二次染色进行双参数分析，已经实现了更具信息性的DNA分析。染色和仪器设备的改进也提高了流式细胞术在“稀有事件”分析中的实用性，即占总细胞悬液不到0.1%至0.5%的细胞类型。已经开发出了基于玻片的商业化图像处理系统，这些系统使用廉价的个人计算机系统提供复杂的细胞和组织分析功能。
