Yao Xiaoyan, Yang Yuzhou, Zhou Zhimin
Biomedical Barriers Research Center, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China.
ACS Omega. 2022 Apr 20;7(17):15064-15073. doi: 10.1021/acsomega.2c00846. eCollection 2022 May 3.
Tissue engineering has made significant progress as a cartilage repair alternative. It is crucial to promote cell proliferation and migration within three-dimensional (3D) bulk scaffolds for tissue regeneration through either chemical gradients or physical channels. In this study, by developing optimized silk fiber-based composite scaffolds, millimeter-scaled channels were created in the corresponding scaffolds via facile physical percussive drilling and subsequently utilized for auricular cartilage regeneration. We found that by the introduction of poly-l-lactic acid porous microspheres (PLLA PMs), the channels incorporated into the (Ap) silk fiber-based scaffolds were reinforced, and the mechanical features were well maintained. Moreover, Ap silk fiber-based scaffolds reinforced by PLLA PMs containing channels (CMAF) exhibited excellent chondrocyte proliferation, migration, and synthesis of cartilage-specific extracellular matrix (ECM) . The biological evaluation revealed that CMAF had a higher chondrogenic capability for an even deposition of the specific ECM component. This study suggested that multihierarchical CMAF may have potential application for auricular cartilage regeneration.
组织工程作为一种软骨修复替代方法已取得显著进展。通过化学梯度或物理通道促进细胞在三维（3D）块状支架内增殖和迁移对于组织再生至关重要。在本研究中，通过开发优化的基于丝纤维的复合支架，通过简便的物理冲击钻孔在相应支架中创建了毫米级通道，并随后将其用于耳廓软骨再生。我们发现，通过引入聚-L-乳酸多孔微球（PLLA PMs），并入基于（Ap）丝纤维的支架中的通道得到增强，并且机械性能得到良好维持。此外，含有通道的PLLA PMs增强的基于Ap丝纤维的支架（CMAF）表现出优异的软骨细胞增殖、迁移以及软骨特异性细胞外基质（ECM）的合成。生物学评估显示，CMAF具有更高的软骨生成能力，可实现特定ECM成分的均匀沉积。本研究表明，多级CMAF可能在耳廓软骨再生中具有潜在应用价值。
