Biomedical Barriers Research Center, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin 300192, China.
Biomedical Barriers Research Center, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin 300192, China; College of Pharmacy & Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
Int J Biol Macromol. 2021 Jul 1;182:1704-1712. doi: 10.1016/j.ijbiomac.2021.05.145. Epub 2021 May 27.
Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics. In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated. We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF). Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF. Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
设计用于耳廓软骨组织工程的临床适用的聚合物复合支架需要具有适当的机械强度和生物特性。在这项研究中，基于丝纤维的支架与聚 L-乳酸多孔微球（PLLA PMs）共增强，同时结合家蚕（Bm）或柞蚕（Ap）丝纤维，这是受到建筑中“钢筋混凝土”结构的启发，并研究了它们的软骨生成功能。我们发现，与 PLLA PMs 增强的家蚕丝纤维支架（MBF）相比，Ap 丝纤维增强的 PLLA PMs 支架（MAF）具有更优异的物理性能（特别是力学性能）。此外，体外软骨生成潜能评估表明，MAF 比 MBF 提供了更好的细胞黏附、活力、增殖和 GAG 分泌。因此，MAF 在耳廓软骨组织工程和相关的整形手术相关应用中具有广阔的应用前景。
