Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
Biomed Mater. 2020 Dec 17;16(1):015025. doi: 10.1088/1748-605X/abca11.
Cartilage defects are among the most difficult diseases to cure in clinic. Due to the limited regeneration capacity of chondrocytes, cartilage regeneration is very difficult. Tissue engineering is a potential strategy for cartilage regeneration. The choice of scaffold is a key factor for the successful construction of tissue engineering cartilage. In this research, we successfully constructed the silk/silk fibroin/gelatin/polylactic acid porous microspheres (S/SF/G/PLLA-PMs) scaffold, then further evaluated the physical and chemical properties and biocompatibility of the composite cartilage tissue in vitro and in vivo, also the long-term survival of the composite cartilage in large animals was carried out. The research results showed that S/SF/G/PLLA-PMs composite scaffold had good biocompatibility. The addition of L-polylactic acid porous microspheres (PLLA-PMs) could significantly enhance the mechanical strength of the scaffold and achieve a multi-level pore structure. After 4 weeks of culture in vitro, composite cartilage could be constructed. Further immunohistochemical results showed that S/SF/G/PLLA-PMs scaffold could increase the long-term stability of the composite cartilage transplantation in vivo.
软骨缺损是临床上最难治愈的疾病之一。由于软骨细胞的再生能力有限，因此软骨再生非常困难。组织工程是软骨再生的一种潜在策略。支架的选择是组织工程软骨成功构建的关键因素。在这项研究中，我们成功构建了丝/丝素/明胶/聚乳酸多孔微球（S/SF/G/PLLA-PMs）支架，然后进一步评估了复合软骨组织在体外和体内的理化性质和生物相容性，以及复合软骨在大动物体内的长期存活情况。研究结果表明，S/SF/G/PLLA-PMs 复合支架具有良好的生物相容性。添加 L-聚乳酸多孔微球（PLLA-PMs）可以显著提高支架的机械强度，并实现多层次的孔结构。体外培养 4 周后，可构建复合软骨。进一步的免疫组织化学结果表明，S/SF/G/PLLA-PMs 支架可提高体内复合软骨移植的长期稳定性。
