Li Bin, Shi Xue-Dong
Department of Urology, Yuyao People's Hospital of Zhejiang Province, Ningbo, Zhejiang, 315400, People's Republic of China.
Int J Gen Med. 2022 Mar 5;15:2515-2527. doi: 10.2147/IJGM.S357013. eCollection 2022.
To investigate the significance of lysosomal protein transmembrane 5 (LAPTM5) in kidney renal clear cell carcinoma (KIRC).
Bioinformatics analysis as an efficient and accurate method was employed to explore the expression levels, prognostic significance, and regulatory pathways of LAPTM5 in KIRC. Finally, the association of LAPTM5 with tumor immune infiltrates was initially investigated.
High LAPTM5 expression was observed in KIRC, and its mRNA expression was correlated with gender, stage, and grade (all < 0.05) but regardless of age. Besides, high LAPTM5 mRNA expression predicted poor overall survival (OS) of KIRC patients ( < 0.01). Further, Cox regression analysis revealed the independent prognostic value of LAPTM5 for OS in KIRC patients ( < 0.001). In addition, the genetic alteration frequency of LAPTM5 was low and had no significant impact on KIRC patient prognosis. However, the low methylation levels of the two methylated sites in the LAPTM5 gene was closely linked to poor OS (all < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA) results showed that the common regulatory pathway was immune- and inflammatory-related pathway. Moreover, LAPTM5 was also associated with tumor immune infiltrates (all < 0.001).
LAPTM5 served as an independent prognostic factor for KIRC patients. LAPTM5 might affect the OS of KIRC patients through the involvement of the immune-related pathway. Therefore, LAPTM5 served as a potential biomarker for OS of KIRC patients.
探讨溶酶体蛋白跨膜5（LAPTM5）在肾透明细胞癌（KIRC）中的意义。
采用生物信息学分析这一高效准确的方法，探究LAPTM5在KIRC中的表达水平、预后意义及调控途径。最后，初步研究LAPTM5与肿瘤免疫浸润的相关性。
在KIRC中观察到LAPTM5高表达，其mRNA表达与性别、分期和分级相关（均<0.05），但与年龄无关。此外，LAPTM5 mRNA高表达预示KIRC患者总生存期（OS）较差（<0.01）。进一步的Cox回归分析显示LAPTM5对KIRC患者的OS具有独立预后价值（<0.001）。此外，LAPTM5的基因改变频率较低，对KIRC患者预后无显著影响。然而，LAPTM5基因中两个甲基化位点的低甲基化水平与较差的OS密切相关（均<0.05）。京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA）结果表明，共同的调控途径是免疫和炎症相关途径。此外，LAPTM5也与肿瘤免疫浸润相关（均<0.001）。
LAPTM5是KIRC患者的独立预后因素。LAPTM5可能通过参与免疫相关途径影响KIRC患者的OS。因此，LAPTM5是KIRC患者OS的潜在生物标志物。
