Attia Ziv, Pogoda Cloe, Vergara Daniela, Kane Nolan C
Ecology and Evolutionary and Biology, University of Colorado, Boulder, 1900 Pleasant Street, Boulder, CO 80302, USA.
AoB Plants. 2021 Oct 30;14(3):plab068. doi: 10.1093/aobpla/plab068. eCollection 2022 Jun.
Currently, the amount of genetic data for is lacking due to the illegal nature of the plant. Our study used 73 whole-genome shotgun libraries to reveal eight different mtDNA haplotypes. The most common haplotype contained 60 of the 73 samples studied and was composed of only dioecious individuals. However, other haplotypes contained a mix of both mating strategies (i.e. monoecious and dioecious). From these haplotype groupings we further examined the fully annotated mitochondrial genomes of four hemp individuals with different mt haplotypes and recorded gene content, copy number variation and synteny. Our results revealed highly syntenic mitochondrial genomes that contained ~60 identifiable sequences for protein-coding genes, tRNAs and rRNAs and no obvious rearrangements or chimeric genes. We found no clear evidence that modern reproductive patterns are due to simple cytoplasmic male sterility mutations. It is likely the interaction between nuclear genetic components and the X/Y sex chromosomes that determines reproductive strategy. Additionally, we added 50 % more mitochondrial genomes to the publicly available repository.
目前，由于该植物的非法性质，其遗传数据量不足。我们的研究使用了73个全基因组鸟枪法文库，以揭示8种不同的线粒体DNA单倍型。最常见的单倍型包含所研究的73个样本中的60个，且仅由雌雄异株个体组成。然而，其他单倍型包含两种交配策略（即雌雄同株和雌雄异株）的混合。从这些单倍型分组中，我们进一步研究了四个具有不同线粒体单倍型的大麻个体的完全注释的线粒体基因组，并记录了基因含量、拷贝数变异和共线性。我们的结果揭示了高度共线的线粒体基因组，其中包含约60个可识别的蛋白质编码基因、tRNA和rRNA序列，且没有明显的重排或嵌合基因。我们没有发现明确的证据表明现代繁殖模式是由简单的细胞质雄性不育突变导致的。很可能是核遗传成分与X/Y性染色体之间的相互作用决定了繁殖策略。此外，我们向公开可用的数据库中添加了50%以上的线粒体基因组。
