Zhao Chun Hai, Liu Xiaoyan, Zhan Tong, He Jianlong
Binzhou Polytechnic Binzhou 256603 Shandong China.
Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University Huaian 223300 China
RSC Adv. 2018 Oct 25;8(63):36233-36238. doi: 10.1039/c8ra05936e. eCollection 2018 Oct 22.
In this study, furfural residues were used as a substrate for cellulase production by the fungi . The results indicated that a low pH and the presence of lignin in the furfural residues have an obvious impact on cellulase production by . After pH adjustment, furfural residues could be used for cellulase production by , with a higher filter paper activity (FPA) and a higher activity of CMCase compared to that yielded from furfural residues with pH unadjusted. After being washed with 1.6% (w/v) HO, all of the lignin in the furfural residues was removed, and an FPA of 7.1 FPU ml and a CMCase activity of 3.4 IU ml were obtained in 115 h, while pretreated straw could yield an FPA of 8.0 FPU ml and a CMCase activity of 2.7 IU ml in 160 h. Moreover, after being treated with HO, furfural residues could be used as an inducer in the production of cellulases. With the treated furfural residues added into the medium at the beginning of cultivation, gave the maximum FPA (8.4 FPU ml) and CMCase activity (4.8 IU ml) at 142 h from pretreated straw, which is relatively high for cellulase production compared to that from most other agricultural wastes reported.
在本研究中,糠醛渣被用作真菌生产纤维素酶的底物。结果表明,低pH值以及糠醛渣中木质素的存在对纤维素酶的生产有明显影响。调节pH值后,糠醛渣可用于生产纤维素酶,与未调节pH值的糠醛渣相比,其滤纸活性(FPA)和羧甲基纤维素酶(CMCase)活性更高。用1.6%(w/v)的H₂O₂洗涤后,糠醛渣中的所有木质素被去除,在115小时内获得了7.1 FPU/ml的FPA和3.4 IU/ml的CMCase活性,而预处理秸秆在160小时内可产生8.0 FPU/ml的FPA和2.7 IU/ml的CMCase活性。此外,用H₂O₂处理后,糠醛渣可作为纤维素酶生产的诱导剂。在培养开始时将处理过的糠醛渣添加到培养基中,预处理秸秆在142小时时产生了最高的FPA(8.4 FPU/ml)和CMCase活性(4.8 IU/ml),与报道的大多数其他农业废弃物相比,这在纤维素酶生产中相对较高。