磷酸化甲基丙烯酰胺壳聚糖水凝胶的合成、表征及成骨作用
Synthesis, characterization and osteogenesis of phosphorylated methacrylamide chitosan hydrogels.
作者信息
Yang Huishang, Chen Shenggui, Liu Lei, Lai Chen, Shi Xuetao
机构信息
National Engineering Research Centre for Tissue Restoration and Reconstruction, School of Material Science and Engineering, South China University of Technology Guangzhou 510640 P. R. China
Peking University Shenzhen Institute, Peking University Shenzhen China.
出版信息
RSC Adv. 2018 Oct 25;8(63):36331-36337. doi: 10.1039/c8ra05378b. eCollection 2018 Oct 22.
Phosphorylated biopolymers can induce mineralization, mimic the process of natural bone formation, and have the potential as scaffolds for bone tissue engineering. 2-Methacryloyloxyethyl phosphorylcholine (MPC), a low cytotoxicity phosphorus source, is mainly applied in vascularization and promoting blood compatibility and has been less researched for bone repair. In this study, phosphorylated methacrylamide chitosan (PMAC) hydrogel was prepared by mixing methacrylamide chitosan (MAC) and different mass of MPC with photoinitiator under UV irradiation. A series characterization tests showed that PMAC hydrogels were successful prepared and had a pretty good mineralization ability. Moreover, human fetal osteoblastic (hFOB) cells cultured on PMAC hydrogels exhibited not only highly viability but also the enhanced ALP activity and calcium deposition. The PMAC hydrogels have great potential in bone tissue engineering applications.
磷酸化生物聚合物可诱导矿化，模拟天然骨形成过程，并具有作为骨组织工程支架的潜力。2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）是一种低细胞毒性的磷源，主要应用于血管生成和促进血液相容性，而在骨修复方面的研究较少。在本研究中，通过将甲基丙烯酰胺壳聚糖（MAC）和不同质量的MPC与光引发剂在紫外线照射下混合，制备了磷酸化甲基丙烯酰胺壳聚糖（PMAC）水凝胶。一系列表征测试表明，成功制备了PMAC水凝胶，且其具有良好的矿化能力。此外，在PMAC水凝胶上培养的人胎儿成骨细胞（hFOB）不仅具有高活力，而且碱性磷酸酶（ALP）活性和钙沉积增强。PMAC水凝胶在骨组织工程应用中具有巨大潜力。
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