Department of Orthopedic Surgery, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
Acta Biomater. 2012 Apr;8(4):1430-9. doi: 10.1016/j.actbio.2011.12.031. Epub 2012 Jan 8.
In this work, we have investigated the development of a synthetic hydrogel that contains a negatively charged phosphate group for use as a substrate for bone cell attachment and differentiation in culture. The photoreactive, phosphate-containing molecule, bis(2-(methacryloyloxy)ethyl)phosphate (BP), was incorporated into oligo(polyethylene glycol) fumarate hydrogel and the mechanical, rheological and thermal properties of the resulting hydrogels were characterized. Our results showed changes in hydrogel compression and storage moduli with incorporation of BP. The modification also resulted in decreased crystallinity as recorded by differential scanning calorimetry. Our data revealed that incorporation of BP improved attachment and differentiation of human fetal osteoblast (hFOB) cells in a dose-dependent manner. A change in surface chemistry and mineralization of the phosphate-containing surfaces verified by scanning electron microscopy and energy dispersive X-ray analysis was found to be important for hFOB cell attachment and differentiation. We also demonstrated that phosphate-containing hydrogels support attachment and differentiation of primary bone marrow stromal cells. These findings suggest that BP-modified hydrogels are capable of sustaining attachment and differentiation of both bone marrow stromal cells and osteoblasts that are critical for bone regeneration.
在这项工作中，我们研究了一种合成水凝胶的发展，该水凝胶含有带负电荷的磷酸基团，可作为培养物中骨细胞附着和分化的基质。将光反应性的含磷分子双(2-(甲基丙烯酰氧)乙基)磷酸酯(BP)掺入聚(乙二醇)富马酸水凝胶中，并对所得水凝胶的机械、流变和热性能进行了表征。我们的结果表明，BP 的掺入会改变水凝胶的压缩和储能模量。差示扫描量热法记录到的改性也导致结晶度降低。我们的数据表明，BP 的掺入以剂量依赖的方式改善了人胎成骨细胞（hFOB）的附着和分化。扫描电子显微镜和能谱分析证实，含磷表面的表面化学和矿化的变化对于 hFOB 细胞的附着和分化很重要。我们还证明，含磷水凝胶支持原代骨髓基质细胞的附着和分化。这些发现表明，BP 修饰的水凝胶能够维持骨髓基质细胞和成骨细胞的附着和分化，这对于骨再生至关重要。
