Rugthaworn Prapassorn, Sukatta Udomlak, Sukyai Prakit
Biotechnology of Biopolymers and Bioactive Compounds Special Research Unit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand.
ACS Omega. 2022 Apr 12;7(16):13455-13464. doi: 10.1021/acsomega.1c06221. eCollection 2022 Apr 26.
This study investigated the effect of ultrasound-assisted hydrogen peroxide (HO) pretreatment on sugar cane bagasse (SCB) followed by TISTR 3003 cultivation for lovastatin production under solid-state fermentation (SSF). Optimization of the pretreatment conditions was investigated using a response surface methodology (RSM). Within the range of the selected operating conditions, the optimized values of HO concentration, amplitude, SCB dosage, and sonication time were found to be 2.74%, 83.22 μm, 2.84% and 52.29 min, respectively. The value of 0.9749 indicated that the fitted model is in good agreement with the predicted and actual lovastatin production. On the basis of the optimum conditions, the lovastatin production was 2347.10 ± 17.19 μg/g, which is 2.4 times higher than that under untreated conditions. Scanning electron microscopy (SEM) analysis explored the surface structure of the untreated SCB, which showed a compact rigid structure. In contrast, treated SCB had a rough surface structure and cracks as a result of the pretreatment.
本研究考察了超声辅助过氧化氢（HO）预处理对甘蔗渣（SCB）的影响，随后在固态发酵（SSF）条件下采用泰国科学技术研究所3003菌株进行洛伐他汀生产。使用响应面法（RSM）对预处理条件进行了优化研究。在所选操作条件范围内，发现HO浓度、振幅、SCB用量和超声处理时间的优化值分别为2.74%、83.22μm、2.84%和52.29分钟。0.9749的值表明拟合模型与预测的和实际的洛伐他汀产量高度吻合。在最佳条件下，洛伐他汀产量为2347.10±17.19μg/g，比未处理条件下高出2.4倍。扫描电子显微镜（SEM）分析探究了未处理SCB的表面结构，其显示出致密的刚性结构。相比之下，经过预处理的SCB表面结构粗糙且有裂缝。
