Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, CEP 12602-810, Brazil.
Food Chem. 2018 Apr 15;245:786-791. doi: 10.1016/j.foodchem.2017.11.111. Epub 2017 Dec 2.
Sugarcane bagasse (SCB) hydrolysate could be an interesting source for red pigment production by Monascus ruber Tieghem IOC 2225. The influence of different wavelength of light-emitting diode (LED) at 250 μmol.m.s of photon flux density on red pigment production by M. ruber in glucose-based medium was evaluated. Then, SCB hydrolysate was used as carbon source under the previously selected light incidence conditions. In glucose-based medium, the highest pigment production was achieved in fermentation assisted with orange LED light (8.28 UA), white light (8.26 UA) and under dark condition (7.45 UA). By using SCB hydrolysate-based medium, the highest red pigment production (18.71 AU) was achieved under dark condition and the glucose and cellobiose present in the hydrolysate were metabolized. SCB enzymatic hydrolysate was demonstrated to be a promising carbon source for high thermal stability red pigment production (activation energy of 10.5 kcal.mol), turning an interesting alternative for implementation in biorefineries.
甘蔗渣水解液可以作为红曲菌（Monascus ruber Tieghem IOC 2225）生产红色素的一个有趣来源。本研究评估了不同波长的发光二极管（LED）在 250µmol.m.s 光量子通量密度下对葡萄糖基培养基中红曲菌产红色素的影响。然后，在先前选择的光照条件下，将甘蔗渣水解液用作碳源。在葡萄糖基培养基中，在橙色 LED 光（8.28UA）、白光（8.26UA）和黑暗条件下（7.45UA）辅助发酵时，色素产量最高。在使用基于甘蔗渣水解液的培养基时，在黑暗条件下实现了最高的红色素产量（18.71AU），并且水解液中存在的葡萄糖和纤维二糖被代谢。甘蔗渣酶解液被证明是一种很有前途的碳源，可以生产高热稳定性红色素（活化能为 10.5kcal.mol），为在生物炼制厂中的应用提供了一个有趣的选择。
