Qian Tianmei, Wang Pan, Chen Qianqian, Yi Sheng, Liu Qianyan, Wang Hongkui, Wang Shiyuan, Geng Wenqing, Liu Zihao, Li Shiying
Key Laboratory of Neuroregeneration of Jiangsu, Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University Nantong Jiangsu 226001 China
RSC Adv. 2018 Dec 10;8(72):41181-41191. doi: 10.1039/c8ra08298g. eCollection 2018 Dec 7.
Schwann cells (SCs), fibroblasts and macrophages are the main cells in the peripheral nerve stumps. These three types of cell play important roles in regulating the regeneration microenvironment and improving the regeneration effect through a variety of manual interventions. However, the dynamic distribution of these cells during the different stages of peripheral nerve regeneration remain unclear. In this study, we systematically explored the number/ratio and distribution changes of SCs, fibroblasts, and macrophages at different time points after sciatic nerve injury. Moreover, considering that let-7 is an ideal molecule for regulating the regeneration environment, we further studied the entrance and influence of let-7 antagomir on these three main cell types. Collectively, our current study revealed the cell basis of the microenvironment of peripheral nerve regeneration and indicated that let-7 could modify the regenerative microenvironment by regulating the number/ratio and distribution of SCs, fibroblasts, and macrophages. Our study would help to open a new potential therapeutic window for peripheral nerve injury.
施万细胞（SCs）、成纤维细胞和巨噬细胞是周围神经残端中的主要细胞。这三种类型的细胞在通过各种人工干预调节再生微环境和提高再生效果方面发挥着重要作用。然而，这些细胞在周围神经再生不同阶段的动态分布仍不清楚。在本研究中，我们系统地探究了坐骨神经损伤后不同时间点施万细胞、成纤维细胞和巨噬细胞的数量/比例及分布变化。此外，鉴于let-7是调节再生环境的理想分子，我们进一步研究了let-7拮抗剂对这三种主要细胞类型的作用及影响。总体而言，我们当前的研究揭示了周围神经再生微环境的细胞基础，并表明let-7可通过调节施万细胞、成纤维细胞和巨噬细胞的数量/比例及分布来改变再生微环境。我们的研究将有助于为周围神经损伤开辟一个新的潜在治疗窗口。
