Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Int J Biol Sci. 2017 Nov 2;13(12):1507-1519. doi: 10.7150/ijbs.21976. eCollection 2017.
Combinations of fibroblasts (Fbs) and corresponding epithelial cells have been widely used in many tissues, such as the skin and breast tissues, to augment tissue repair and remodeling. Recently, a large amount of new data has indicated that nerve Fbs play critical roles in Schwann cells (SCs) and axons . However, little is known regarding the effects of co-transplanting nerve Fbs and SCs on peripheral nerve repair . The aim of this study was to investigate the effect of co-transplanting sciatic nerve Fbs (SN-Fbs) and sciatic nerve SCs (SN-SCs) on nerve regeneration. We developed a 5 mm nerve-defect model in mice using a polyurethane (PUR) catheter and then injected one of four different mixtures of cells into the catheters to form the following four groups: pure Matrigel (Control group), SN-Fbs alone (SN-Fb group), SN-Fbs combined with SN-SCs at a ratio of 1:2 (Fb&SC group) and SN-SCs alone (SN-SC group). Histological and functional analyses were performed 3 months later. The results indicated that , the expression levels of NGF, BDNF and GDNF were significantly higher, and , a more moderate amount of extracellular matrix was produced in the Fb&SC group than in the SN-SC group. Compared to the other groups, co-transplanting SN-Fbs with SCs at a 1:2 ratio had significantly positive effects on nerve regeneration and functional recovery.
成纤维细胞（Fbs）和相应的上皮细胞的组合已被广泛应用于许多组织，如皮肤和乳腺组织，以增强组织修复和重塑。最近，大量新数据表明，神经成纤维细胞在 Schwann 细胞（SCs）和轴突中发挥关键作用。然而，关于共移植神经成纤维细胞和SCs 对周围神经修复的影响知之甚少。本研究旨在探讨共移植坐骨神经成纤维细胞（SN-Fbs）和坐骨神经SCs（SN-SCs）对神经再生的影响。我们使用聚氨酯（PUR）导管在小鼠中建立了 5mm 的神经缺损模型，然后将四种不同细胞混合物之一注入导管中，形成以下四组：纯 Matrigel（对照组）、单独的 SN-Fbs（SN-Fb 组）、SN-Fbs 与 SN-SCs 以 1:2 的比例组合（Fb&SC 组）和单独的 SN-SCs（SN-SC 组）。3 个月后进行组织学和功能分析。结果表明，Fb&SC 组的 NGF、BDNF 和 GDNF 表达水平显著升高，且细胞外基质的产生量适中。与其他组相比，以 1:2 的比例共移植 SN-Fbs 和SCs 对神经再生和功能恢复有显著的积极影响。
