Li Guang-Shuai, Li Qing-Feng, Dong Ming-Min, Zan Tao, Ding Shuang, Liu Lin-Bo
Plastic & Reconstructive Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
Plastic & Reconstructive Surgery of the Ninth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.
Neural Regen Res. 2016 Apr;11(4):682-6. doi: 10.4103/1673-5374.180758.
Nerve regeneration conditioned fluid is secreted by nerve stumps inside a nerve regeneration chamber. A better understanding of the proteinogram of nerve regeneration conditioned fluid can provide evidence for studying the role of the microenvironment in peripheral nerve regeneration. In this study, we used cylindrical silicone tubes as the nerve regeneration chamber model for the repair of injured rat sciatic nerve. Isobaric tags for relative and absolute quantitation proteomics technology and western blot analysis confirmed that there were more than 10 complement components (complement factor I, C1q-A, C1q-B, C2, C3, C4, C5, C7, C8β and complement factor D) in the nerve regeneration conditioned fluid and each varied at different time points. These findings suggest that all these complement components have a functional role in nerve regeneration.
神经再生条件培养液由神经再生室内的神经残端分泌。更好地了解神经再生条件培养液的蛋白质图谱可为研究微环境在周围神经再生中的作用提供证据。在本研究中，我们使用圆柱形硅胶管作为神经再生室模型来修复大鼠坐骨神经损伤。相对和绝对定量蛋白质组学技术的等压标签和蛋白质印迹分析证实，神经再生条件培养液中存在10多种补体成分（补体因子I、C1q-A、C1q-B、C2、C3、C4、C5、C7、C8β和补体因子D），且每种成分在不同时间点有所变化。这些发现表明，所有这些补体成分在神经再生中都具有功能性作用。
