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剪切均质辅助提取法从菠萝皮渣中提取可溶性膳食纤维的优化及其功能特性

Optimization extraction and functional properties of soluble dietary fiber from pineapple pomace obtained by shear homogenization-assisted extraction.

作者信息

Hu Huigang, Zhao Qiaoli

机构信息

Key Laboratory of Tropical Fruit Tree Biology, Ministry of Agriculture, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences Zhanjiang China

出版信息

RSC Adv. 2018 Dec 7;8(72):41117-41130. doi: 10.1039/c8ra06928j.

Abstract

Response surface methodology (RSM) was used to optimize the extraction conditions for shear homogenization-assisted extraction of soluble dietary fiber from pineapple pomace (s-SDF), and the absorption capacities and antioxidant activities of the obtained s-SDF were also investigated. The optimum extraction conditions consisted of a cutting speed of 9000 rpm, a cutting time of 20 min, a cellulase content of 5.0%, a hydrolysis time of 120 min, a pH value of 4.5, a hydrolysis temperature of 50 °C, and a raw material to water ratio of 1 : 45 g mL. Under these conditions, the theoretical and actual extraction yields of s-SDF were 8.80% and 8.76%, respectively. An absorption capacity analysis indicated that s-SDF exhibited higher absorption abilities to sodium cholate, cholesterol and fat. In addition, s-SDF possessed higher antioxidant activities, showing a positive concentration effect relationship for DPPH˙, ABTS, ·OH and O ˙. The concentration of 1.0 mg mL scavenged 76.72% DPPH˙, 58.40% ABTS, 23.47% ·OH and 48.47% O ˙, respectively, and the reduction power was 0.70. These results indicated that pineapple pomace is a potential source of natural dietary fiber and a potential functional food ingredient.

摘要

采用响应面法(RSM)优化菠萝皮渣中可溶性膳食纤维(s-SDF)的剪切均质辅助提取条件,并研究所得s-SDF的吸附能力和抗氧化活性。最佳提取条件包括切割速度9000转/分钟、切割时间20分钟、纤维素酶含量5.0%、水解时间120分钟、pH值4.5、水解温度50℃以及原料与水的比例为1:45克/毫升。在此条件下,s-SDF的理论提取率和实际提取率分别为8.80%和8.76%。吸附能力分析表明,s-SDF对胆酸钠、胆固醇和脂肪具有较高的吸附能力。此外,s-SDF具有较高的抗氧化活性,对DPPH˙、ABTS、·OH和O˙呈现正浓度效应关系。浓度为1.0毫克/毫升时,分别清除了76.72%的DPPH˙、58.40%的ABTS、23.47%的·OH和48.47%的O˙,还原力为0.70。这些结果表明,菠萝皮渣是天然膳食纤维的潜在来源和潜在的功能性食品成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db3/9092029/729ef8422d32/c8ra06928j-f1.jpg

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