Hu Huigang, Zhao Qiaoli
Key Laboratory of Tropical Fruit Tree Biology, Ministry of Agriculture, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences Zhanjiang China
RSC Adv. 2018 Dec 7;8(72):41117-41130. doi: 10.1039/c8ra06928j.
Response surface methodology (RSM) was used to optimize the extraction conditions for shear homogenization-assisted extraction of soluble dietary fiber from pineapple pomace (s-SDF), and the absorption capacities and antioxidant activities of the obtained s-SDF were also investigated. The optimum extraction conditions consisted of a cutting speed of 9000 rpm, a cutting time of 20 min, a cellulase content of 5.0%, a hydrolysis time of 120 min, a pH value of 4.5, a hydrolysis temperature of 50 °C, and a raw material to water ratio of 1 : 45 g mL. Under these conditions, the theoretical and actual extraction yields of s-SDF were 8.80% and 8.76%, respectively. An absorption capacity analysis indicated that s-SDF exhibited higher absorption abilities to sodium cholate, cholesterol and fat. In addition, s-SDF possessed higher antioxidant activities, showing a positive concentration effect relationship for DPPH˙, ABTS, ·OH and O ˙. The concentration of 1.0 mg mL scavenged 76.72% DPPH˙, 58.40% ABTS, 23.47% ·OH and 48.47% O ˙, respectively, and the reduction power was 0.70. These results indicated that pineapple pomace is a potential source of natural dietary fiber and a potential functional food ingredient.
采用响应面法(RSM)优化菠萝皮渣中可溶性膳食纤维(s-SDF)的剪切均质辅助提取条件,并研究所得s-SDF的吸附能力和抗氧化活性。最佳提取条件包括切割速度9000转/分钟、切割时间20分钟、纤维素酶含量5.0%、水解时间120分钟、pH值4.5、水解温度50℃以及原料与水的比例为1:45克/毫升。在此条件下,s-SDF的理论提取率和实际提取率分别为8.80%和8.76%。吸附能力分析表明,s-SDF对胆酸钠、胆固醇和脂肪具有较高的吸附能力。此外,s-SDF具有较高的抗氧化活性,对DPPH˙、ABTS、·OH和O˙呈现正浓度效应关系。浓度为1.0毫克/毫升时,分别清除了76.72%的DPPH˙、58.40%的ABTS、23.47%的·OH和48.47%的O˙,还原力为0.70。这些结果表明,菠萝皮渣是天然膳食纤维的潜在来源和潜在的功能性食品成分。