Natural Medicine Research Center, College of veterinary medicine, Sichuan Agricultural University, Chengdu 611130, PR China.
Natural Medicine Research Center, College of veterinary medicine, Sichuan Agricultural University, Chengdu 611130, PR China.
Int J Biol Macromol. 2018 Nov;119:70-78. doi: 10.1016/j.ijbiomac.2018.07.131. Epub 2018 Jul 20.
Four polysaccharides, PTP-1, PTP-2, PTP-3 and PTP-4, were obtained from the water extraction of the stems of P. tricuspidata by anion exchange chromatography and gel filtration. The antioxidant activities of four PTPs were investigated, exhibiting different antioxidant activities, in which PTP-4 performed noticeable, with strong superoxide radical activity (comparable to BHT), high DPPH radical activity (78.53% at 1250 μg/mL), moderate hydroxyl radical scavenging activity and reducing power activity. Furthermore, the chemical structure of PTP-4 was measured by FT-IR, GC, H and C NMR spectra, indicating its mainly composition of the arabinose, xylose, galactose, glucuronic acid, and mannose. Thus, the stems of P. tricuspidata could be used as a potential source for natural antioxidant.
四种多糖，PTP-1、PTP-2、PTP-3 和 PTP-4，从三脉紫菀茎的水提物中通过阴离子交换层析和凝胶过滤获得。研究了四种 PTP 的抗氧化活性，表现出不同的抗氧化活性，其中 PTP-4 表现出显著的超氧自由基活性（与 BHT 相当）、高 DPPH 自由基活性（在 1250μg/mL 时为 78.53%）、适度的羟基自由基清除活性和还原能力。此外，通过傅里叶变换红外光谱（FT-IR）、气相色谱（GC）、氢谱（H NMR）和碳谱（C NMR）对 PTP-4 的化学结构进行了测定，表明其主要由阿拉伯糖、木糖、半乳糖、葡萄糖醛酸和甘露糖组成。因此，三脉紫菀的茎可作为天然抗氧化剂的潜在来源。
