Colorado Center for Reproductive Medicine, Lone Tree, Colorado.
Colorado Center for Reproductive Medicine, Lone Tree, Colorado; Department of Animal Science, University of Nebraska - Lincoln, Lincoln, Nebraska.
F S Sci. 2021 Feb;2(1):50-58. doi: 10.1016/j.xfss.2020.12.006. Epub 2020 Dec 30.
To characterize fatty acid (FA) profile of commercially available albumin products and determine their effect on embryonic development.
Research study.
Private research facility.
ANIMAL(S): Outbred mice aged 4-8 weeks.
INTERVENTION(S): Gas chromatography-mass spectrometry was used to quantify the FA content of 15 commercial albumins. Embryos were produced in media containing different albumin products, with or without carnitine or exogenous FA supplementation, to determine their effect on embryo development in vitro.
MAIN OUTCOME MEASURE(S): Total micrograms of FA per milligram of albumin for the 15 albumin products, blastocyst development, cell number, allocation to the trophectoderm (TE) or inner cell mass (ICM), and evaluation of morphology during implantation.
RESULT(S): The albumin products contained 0.07-16.77 μg total FA/mg albumin. Compared to media with with >1.4 μg FA/mg albumin, media with <0.5 μg FA/mg albumin supported improved blastocyst development, and addition of carnitine mitigated this difference. Addition of palmitoleic acid or oleic acid individually did not improve blastocyst development and decreased ICM:TE ratio. However, in the presence of carnitine, there was improved blastocyst development and maintenance of the ICM:TE ratio. Embryos cultured in Vitrolife human serum albumin with supplementation of carnitine, palmitoleic acid, and oleic acid were more likely to develop cells positive for POU5F1 in an extended embryo culture than embryos cultured in Origio serum protein substitute.
CONCLUSION(S): Commercial albumin products contain FAs, which vary in abundance. These FAs have different effects on embryo development and quality before and during the implantation stage. Several of these albumin preparations are routinely used for human-assisted reproductive technologies; therefore, serious consideration is warranted when selecting a product for clinical use.
描述市售白蛋白产品的脂肪酸（FA）谱，并确定其对胚胎发育的影响。
研究。
私人研究设施。
年龄为 4-8 周的近交系小鼠。
气相色谱-质谱联用技术用于定量分析 15 种商业白蛋白产品中的 FA 含量。胚胎在含有不同白蛋白产品的培养基中产生，有或没有肉碱或外源性 FA 补充，以确定它们对体外胚胎发育的影响。
15 种白蛋白产品每毫克白蛋白的总 FA 微克数，囊胚发育，细胞数，分配给滋养外胚层（TE）或内细胞团（ICM），以及着床期间形态学评价。
白蛋白产品含有 0.07-16.77 μg 总 FA/mg 白蛋白。与 FA/mg 白蛋白>1.4 μg 的培养基相比，FA/mg 白蛋白<0.5 μg 的培养基支持囊胚发育改善，添加肉碱可减轻这种差异。单独添加棕榈油酸或油酸均不能改善囊胚发育并降低 ICM:TE 比。然而，在肉碱存在的情况下，囊胚发育得到改善，ICM:TE 比得以维持。在补充肉碱、棕榈油酸和油酸的 Vitrolife 人血清白蛋白中培养的胚胎比在 Origio 血清蛋白替代物中培养的胚胎更有可能在延长的胚胎培养中发育出对 POU5F1 呈阳性的细胞。
商业白蛋白产品含有 FA，其丰度不同。这些 FA 对胚胎发育和着床前及着床期的胚胎质量有不同的影响。这些白蛋白制剂中的几种常用于人类辅助生殖技术；因此，在选择用于临床使用的产品时，应认真考虑。
