Biological & Agricultural Engineering, Louisiana State University and LSU Ag Center, United States of America.
Connecticut Agricultural Experiment Station, United States of America.
NanoImpact. 2021 Jul;23:100329. doi: 10.1016/j.impact.2021.100329. Epub 2021 Jun 1.
The majority of published research on the effect of engineered nanoparticles on terrestrial plant species is focused on inorganic nanoparticles, with the effects of organic polymeric nanoparticles (NP) on plants remaining largely unexplored. It is critical to understand the impact of polymeric NPs on plants if these particles are to be used as agrochemical delivery systems. This study investigates the effect of biodegradable polymeric lignin-based nanoparticles (LNPs) and zein nanoparticles (ZNP) on soybean plant health. The LNPs (114 ± 3.4 nm, -53.8 ± 6.9 mV) were synthesized by emulsion evaporation from lignin-graft-poly(lactic-co-glycolic) acid, and ZNPs (142 ± 3.9 nm and + 64.5 ± 4.7 mV) were synthesized by nanoprecipitation. Soybeans were grown hydroponically and treated with 0.02, 0.2, and 2 mg/ml of LNPs or ZNPs at 28 days after germination. Plants were harvested after 1, 3, 7 and 14 days of particle exposure and analyzed for root and stem length, chlorophyll concentration, dry biomass of roots and stem, nutrient uptake and plant ROS. Root and stem length, chlorophyll and stem biomass did not differ significantly between treatments and controls for LNPs-treated plants at all concentrations, and at low doses of ZNPs. At 2 mg/ml ZNPs, the highest concentration tested, after 7 days of treatment chlorophyll levels and root biomass increased and stem length was reduced in comparison to the control. Nutrient uptake was largely unaffected at 0.02 and 0.2 mg/ml NPs. A concentration-dependent increase in the oxidative stresss was detected, especially in the ZNP treated plants. Overall, LNPs and ZNPs had a minimum impact on soybean health especially at low and medium doses. To our knowledge this is the first study to show the effect of zein and lignin based polymeric NPs designed for agrochemical delivery on soybean plant health.
大多数关于工程纳米颗粒对陆生植物物种影响的已发表研究都集中在无机纳米颗粒上，而对于有机聚合物纳米颗粒（NP）对植物的影响则在很大程度上尚未得到探索。如果要将这些颗粒用作农用化学品输送系统，了解聚合物 NP 对植物的影响至关重要。本研究调查了可生物降解聚合物木质素基纳米颗粒（LNPs）和玉米醇溶蛋白纳米颗粒（ZNP）对大豆植物健康的影响。LNPs（114 ± 3.4nm，-53.8 ± 6.9mV）通过从木质素接枝聚（乳酸-共- 乙交酯）的乳液蒸发合成，ZNPs（142 ± 3.9nm 和 +64.5 ± 4.7mV）通过纳米沉淀合成。大豆通过水培生长，并在发芽后 28 天用 0.02、0.2 和 2mg/ml 的 LNPs 或 ZNPs 处理。在颗粒暴露 1、3、7 和 14 天后收获植物，并分析根和茎长、叶绿素浓度、根和茎的干生物量、养分吸收和植物 ROS。在所有浓度下，LNPs 处理的植物的根和茎长、叶绿素和茎生物量与对照相比均无显著差异，并且在 ZNP 的低剂量下也无显著差异。在 2mg/ml ZNPs 的最高测试浓度下，与对照相比，在 7 天的处理后，叶绿素水平和根生物量增加，而茎长降低。在 0.02 和 0.2mg/ml NPs 时，养分吸收基本不受影响。检测到氧化应激呈浓度依赖性增加，尤其是在 ZNP 处理的植物中。总体而言，LNPs 和 ZNPs 对大豆健康的影响很小，尤其是在低剂量和中剂量时。据我们所知，这是第一项研究表明设计用于农用化学品输送的玉米醇溶蛋白和木质素基聚合物 NP 对大豆植物健康的影响。
