Department of Biological and Agricultural Engineering , Louisiana State University Agricultural and Mechanical College and LSU AgCenter , Baton Rouge , Louisiana 70803 , United States.
General and Inorganic Chemistry Department , Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy , 12, Ion Creanga Street , 400010 , Cluj-Napoca , Romania.
J Agric Food Chem. 2018 Jul 5;66(26):6544-6551. doi: 10.1021/acs.jafc.7b02487. Epub 2017 Aug 17.
The main objective of this study was to investigate the uptake and translocation of positively charged zein nanoparticles (ZNPs) in hydroponically grown sugar cane plants. Fluorescent ZNPs (spherical and measuring an average diameter 135 ± 3 nm) were synthesized by emulsion-diffusion method from FITC-tagged zein. Fluorescent measurement following digestion of plant tissue indicated that sugar cane roots had a significant adhesion of ZNPs, 342.5 ± 24.2 μg NPs/mg of dry matter, while sugar cane leaves contained a very limited amount, 12.9 ± 1.2 μg NPs/mg dry matter for high dose(1.75 mg/ml) after 12 h. Confocal microscopy studies confirmed presence of fluorescent ZNPs in the epidermis and endodermis of the root system. Given their ability to adhere to roots for extended periods of time, ZNPs are proposed as effective delivery systems for agrochemicals to sugar cane plants, but more studies are needed to identify effect of nanoparticle exposure to health of the plant.
本研究的主要目的是研究带正电荷的玉米醇溶蛋白纳米颗粒（ZNP）在水培甘蔗植物中的摄取和转运。通过乳化扩散法从 FITC 标记的玉米醇溶蛋白合成了荧光 ZNP（球形，平均直径为 135±3nm）。植物组织消化后的荧光测量表明，甘蔗根对 ZNP 具有显著的黏附性，每毫克干物质中含有 342.5±24.2μg 的纳米颗粒，而对于高剂量（1.75mg/ml），甘蔗叶中仅含有非常有限的量，12.9±1.2μg NPs/mg 干物质，在 12 小时后。共聚焦显微镜研究证实了荧光 ZNP 存在于根系的表皮和内皮层中。鉴于它们能够长时间黏附在根部，ZNP 被提议作为向甘蔗植物输送农用化学品的有效载体，但需要进一步研究以确定纳米颗粒暴露对植物健康的影响。
