Colorado Center for Reproductive Medicine-Colorado, Lone Tree, Colorado.
Colorado Center for Reproductive Medicine Fertility Network, Lone Tree, Colorado.
F S Sci. 2021 Aug;2(3):268-277. doi: 10.1016/j.xfss.2021.06.005. Epub 2021 Jul 6.
To assess the impact of shipment and storage of sperm, oocytes, and blastocysts in vapor phase nitrogen compared with static storage in liquid phase nitrogen.
Prospective cohort-matched study.
Multiple in vitro fertilization laboratories in an in vitro fertilization network.
PATIENT(S): Fifty-eight human embryos, 32 human oocytes, 15 units of bovine semen.
INTERVENTION(S): Vapor vs. liquid nitrogen.
MAIN OUTCOME MEASURE(S): The postwarming survival of oocytes, sperm, and blastocysts, and the developmental potential of blastocysts during in vitro extended culture.
RESULT(S): Custom-designed labware, for use with the TMRW platform, enables continuous temperature monitoring during shipment and/or storage in the vapor phase robotic storage system. The highest temperature recorded for specimens shipped to a domestic laboratory was -180.2 °C with a mean ± SD of -190.4 ± 0.5 °C during shipment and -181.1 ± 0.6 °C during storage. Likewise, specimens shipped internationally had a high of -180.2 °C with a mean ± SD of -193.5 ± 0.6 °C during shipment and -181.2 ± 0.7 °C during storage. Results from the extended culture assays have revealed no deleterious effect of shipment and storage in nitrogen vapor. The viability of mammalian gametes and embryos was equivalent between the vapor phase and liquid phase storage.
CONCLUSION(S): The evaluated system did not have any deleterious effects on the postwarming survival of sperm, oocytes, and blastocysts. The postwarming developmental potential of human blastocysts during in vitro extended culture was unaffected by storage and handling in the vapor phase nitrogen TMRW platform when compared with static liquid phase nitrogen storage. Our results suggest that the vapor phase cryostorage platform is a safe system to handle and store reproductive specimens for human assisted reproductive technology.
评估与液态氮静态储存相比，将精子、卵子和胚胎在气相氮中运输和储存对其的影响。
前瞻性队列匹配研究。
体外受精网络中的多个体外受精实验室。
58 个人类胚胎、32 个人类卵子、15 个牛精液单位。
气相与液相。
卵子、精子和胚胎解冻后的存活率，以及胚胎在体外延长培养过程中的发育潜能。
定制的实验室设备，用于 TMRW 平台，可在气相机器人储存系统中运输和/或储存过程中进行连续温度监测。运往国内实验室的样本的最高记录温度为-180.2°C，平均（±SD）为-190.4°C±0.5°C 运输过程中-181.1°C±0.6°C 储存期间。同样，运往国际的样本最高温度为-180.2°C，平均（±SD）为-193.5°C±0.6°C 运输过程中-181.2°C±0.7°C 储存期间。延长培养试验的结果表明，氮气相运输和储存没有产生有害影响。哺乳动物配子和胚胎的活力在气相和液相储存之间是等效的。
评估的系统对精子、卵子和胚胎解冻后的存活率没有任何不良影响。与静态液态氮储存相比，在体外延长培养过程中，人类胚胎解冻后的发育潜能在气相氮 TMRW 平台的运输和储存过程中不受影响。我们的结果表明，气相低温储存平台是一种安全的系统，可以用于处理和储存人类辅助生殖技术的生殖标本。
