Laboratory of Functional Physiology and Bio-resources Valorisation, Higher Institute of Biotechnology of Beja, University of Jendouba, Beja, Tunisia.
Laboratory of Natural Substances, National Institute for Research and Physico-Chemical Analysis (INRAP), Technopole Sidi Thabet, Technological Pole, Ariana, Tunisia.
J Med Food. 2022 May;25(5):513-522. doi: 10.1089/jmf.2020.0217.
(Fenugreek) is used in traditional phytomedicine for its anti-inflammatory, antiseptic, antidiabetic, and several other therapeutic virtues. The current study was intended to investigate the protecting effects of fenugreek seeds' aqueous extract (FSAE) using experimentally ethanol (EtOH)-induced gastric peptic ulcer in rats, as immense alcohol consumption can lead to gastric ulcer. Sixty adult male Wistar rats were divided into 6 groups of 10 each: control, EtOH (4 g/kg body weight [b.w.]), EtOH + several doses of FSAE (50, 100, and 200 mg/kg b.w.), and EtOH + Omeprazole (OM, 20 mg/kg orally []). Animals were pretreated with FSAE for 21 days and exposed to a single oral administration of EtOH (4 g/kg b.w.) for 2 h. Gastric ulcer in rats was induced with a single dose of EtOH. Ulcer index, malondialdehyde (MDA), hydrogen peroxide (HO), and thiol groups (-SH) content in stomach, and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured. Our recorded results showed that EtOH induced gastric damage, evidenced by the level of oxidative stress markers such as MDA and HO in rats exposed to EtOH. However, significant increases in the activities of antioxidant enzymes were recorded, such as SOD, CAT, and GPx, and a decrease in nonenzymatic antioxidants, such as (-SH). Moreover, histopathological examinations showed the presence of lesions associated with severe tissue damage in the untreated rats. Interestingly, FSAE meaningfully protects against all gastric damages caused by EtOH. We propose that FSAE exhibits protective effects in EtOH-induced peptic ulcer in rats. This protection might be related to its antioxidant and anti-inflammatory properties as well as its opposite effects on some studied intracellular mediators.
（葫芦巴）在传统植物药中因其具有抗炎、防腐、抗糖尿病等多种治疗功效而被使用。本研究旨在通过实验性乙醇（EtOH）诱导的大鼠胃溃疡，来研究葫芦巴种子水提物（FSAE）的保护作用，因为大量饮酒可能导致胃溃疡。将 60 只成年雄性 Wistar 大鼠分为 6 组，每组 10 只：对照组、EtOH（4 g/kg 体重）、EtOH+几种剂量的 FSAE（50、100 和 200 mg/kg b.w.）和 EtOH+奥美拉唑（OM，20 mg/kg 口服）。动物用 FSAE 预处理 21 天，然后给予单次口服 EtOH（4 g/kg b.w.）2 小时。用单次剂量 EtOH 诱导大鼠胃溃疡。测量大鼠胃中的溃疡指数、丙二醛（MDA）、过氧化氢（HO）和巯基（-SH）含量以及抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）。我们的记录结果表明，EtOH 诱导了胃损伤，这可以从暴露于 EtOH 的大鼠的氧化应激标志物 MDA 和 HO 的水平中得到证明。然而，记录到抗氧化酶的活性显著增加，如 SOD、CAT 和 GPx，以及非酶抗氧化剂如（-SH）的减少。此外，组织病理学检查显示，未经处理的大鼠存在与严重组织损伤相关的病变。有趣的是，FSAE 对 EtOH 引起的所有胃损伤都有显著的保护作用。我们提出 FSAE 在 EtOH 诱导的大鼠胃溃疡中表现出保护作用。这种保护可能与其抗氧化和抗炎特性以及对一些研究的细胞内介质的相反作用有关。
