Department of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Research Center in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Nutrients. 2021 Dec 30;14(1):172. doi: 10.3390/nu14010172.
Phy-Blica-D is a traditional Thai polyherbal formula that has reduced oxidative stress in non-communicable diseases. However, evidence supporting the gastroprotective effects of Phy-Blica-D has not been previously reported. Therefore, this study aimed to evaluate the gastroprotective effects of Phy-Blica-D against gastric ulcers in rats and investigate the potential underlying mechanism. To estimate the possible mechanisms of action, we examined the levels of oxidative stress markers, such as reactive oxygen species (ROS) and malondialdehyde (MDA), as well as antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH). According to our results, rats treated with only 80% ethanol (vehicle group) exhibited significant increases in their ulcer area and ulcer index (UI). Moreover, the levels of ROS and MDA markedly increased in the vehicle group compared with the normal control group. Daily oral administration of Phy-Blica-D (500 and 1000 mg/kg) for 7 days not only significantly decreased the ulcer area and UI, but also remarkably decreased the ROS and MDA levels in gastric tissue. Gastric ulcers induced by ethanol had significantly decreased antioxidant enzyme activities (CAT and SOD) and non-enzymatic antioxidant (GSH), whereas pretreatment with Phy-Blica-D significantly improved the activities of CAT, SOD, and GSH. Moreover, after exposure to ethanol, the rats exhibited a significantly increased level of inducible nitric oxide synthase (iNOS), which was reduced after treatment with Phy-Blica-D. These findings suggest that Phy-Blica-D potentially exerts its gastroprotective effects by suppressing oxidative stress and stimulating antioxidant enzymes, which is one of the causes of destruction of cell membranes, and it is involved in the pathogenesis of acute gastric ulcers induced by ethanol.
Phy-Blica-D 是一种传统的泰草药配方，已被证明可减轻非传染性疾病中的氧化应激。然而，先前尚未有研究报道 Phy-Blica-D 具有胃保护作用。因此，本研究旨在评估 Phy-Blica-D 对大鼠胃溃疡的胃保护作用，并探讨其潜在的作用机制。为了评估可能的作用机制，我们检测了氧化应激标志物的水平，如活性氧（ROS）和丙二醛（MDA），以及抗氧化酶，包括过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）。根据我们的结果，仅用 80%乙醇（载体组）处理的大鼠其溃疡面积和溃疡指数（UI）显著增加。此外，与正常对照组相比，载体组的 ROS 和 MDA 水平明显升高。每天口服 Phy-Blica-D（500 和 1000mg/kg）连续 7 天不仅显著降低了溃疡面积和 UI，而且还显著降低了胃组织中的 ROS 和 MDA 水平。乙醇诱导的胃溃疡导致抗氧化酶活性（CAT 和 SOD）和非酶抗氧化剂（GSH）显著降低，而 Phy-Blica-D 预处理可显著提高 CAT、SOD 和 GSH 的活性。此外，在接触乙醇后，大鼠诱导型一氧化氮合酶（iNOS）水平显著升高，而 Phy-Blica-D 处理后其水平降低。这些结果表明，Phy-Blica-D 通过抑制氧化应激和刺激抗氧化酶来发挥其胃保护作用，这是破坏细胞膜的原因之一，并且涉及到乙醇诱导的急性胃溃疡的发病机制。
