Thirunavukkarasu V, Anuradha C V, Viswanathan P
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu, India.
Phytother Res. 2003 Aug;17(7):737-43. doi: 10.1002/ptr.1198.
The study investigates the effect of aqueous extract of fenugreek seeds (Trigonella foenum graecum) on lipid peroxidation and antioxidant status in experimental ethanol toxicity in rats. The ability of the seed extract to prevent iron-induced lipid peroxidation in vitro was also investigated. Ethanol feeding for 60 days resulted in significant increases in the activities of serum aspartate transaminase, alanine transaminase and alkaline phosphatase. The levels of serum lipid hydroperoxides and thiobarbituric acid reactive substances in liver and brain were also significantly elevated. Significantly lower activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and glutathione reductase were observed in liver and brain accompanied by depletion in glutathione, ascorbic acid and alpha-tocopherol concentrations. Activity of Ca(2+) ATPase in brain was significantly lowered. Simultaneous administration of aqueous extract of fenugreek seeds with ethanol prevented the enzymatic leakage and the rise in lipid peroxidation and enhanced the antioxidant potential. The seeds exhibited appreciable antioxidant property in vitro which was comparable with that of reduced glutathione and alpha-tocopherol. Further, histopathological examination of liver and brain revealed that, aqueous extract of fenugreek seeds could offer a significant protection against ethanol toxicity.
本研究调查了胡芦巴种子水提取物对大鼠实验性乙醇中毒中脂质过氧化和抗氧化状态的影响。还研究了种子提取物在体外预防铁诱导的脂质过氧化的能力。喂食乙醇60天导致血清天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶的活性显著增加。肝脏和大脑中血清脂质氢过氧化物和硫代巴比妥酸反应性物质的水平也显著升高。在肝脏和大脑中观察到超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶和谷胱甘肽还原酶的活性显著降低，同时谷胱甘肽、抗坏血酸和α-生育酚浓度减少。大脑中Ca(2+)ATP酶的活性显著降低。胡芦巴种子水提取物与乙醇同时给药可防止酶泄漏和脂质过氧化的增加，并增强抗氧化潜力。种子在体外表现出明显的抗氧化特性，与还原型谷胱甘肽和α-生育酚相当。此外，肝脏和大脑的组织病理学检查显示，胡芦巴种子水提取物可以提供显著的抗乙醇毒性保护。
